Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5357824..5358449 Replicon chromosome
Accession NZ_CP061404
Organism Klebsiella pneumoniae strain NU-CRE055

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HCV81_RS26520 Protein ID WP_002882817.1
Coordinates 5357824..5358207 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HCV81_RS26525 Protein ID WP_004150355.1
Coordinates 5358207..5358449 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV81_RS26505 5355190..5356092 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HCV81_RS26510 5356089..5356724 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HCV81_RS26515 5356721..5357650 + 930 WP_059065082.1 formate dehydrogenase accessory protein FdhE -
HCV81_RS26520 5357824..5358207 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HCV81_RS26525 5358207..5358449 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HCV81_RS26530 5358654..5359571 + 918 WP_059065084.1 alpha/beta hydrolase -
HCV81_RS26535 5359585..5360526 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HCV81_RS26540 5360571..5361008 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HCV81_RS26545 5361005..5361865 - 861 WP_004146232.1 virulence factor BrkB family protein -
HCV81_RS26550 5361859..5362458 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T172645 WP_002882817.1 NZ_CP061404:c5358207-5357824 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172645 NZ_CP079837:c4235686-4235333 [Salmonella enterica subsp. salamae]
GTGTGGACGATTAAAACAACGGATACGTTTGATCGCTGGTTTACCTCGCTCAACGATACCGACAGGGCCAGCGCGCTTGC
AGCGCTGTTGGTCCTGCGGGAAAAGGGGCCGGGTTTGTCCAGACCTTACGCCGATACACTCAGAGGCTCCCGCTACAGCA
ACATGAAAGAGCTGCGCATTCAGAGCCGGGGCGAGCCGATACGGGCGTTTTTCGCTTTTGATCCGACCCGCACCGGCATT
GTGCTTTGTGCCGGAAATAAGGTCGGCAATGAAAAACGTTTTTACGATGAGATGCTCCCGGTCGCTGACCGGGAATTCAC
GAACTGGCTGAACACATTAAAAGAGAAGGAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172645 WP_004150355.1 NZ_CP061404:c5358449-5358207 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172645 NZ_CP079837:c4235328-4235026 [Salmonella enterica subsp. salamae]
ATGGGCAGAACGCTGGAACAGCTTATTGCGGATGAGAAACCTGAAGTCGTGGCCGAAGCTCAGGCGATGGCGATGGATAT
CCTGCTCAACATTCACCTTGCCGAACTGCGTGAGAAGGTACAGAAAACTCAGGTTGAAATGGCGCAGGCGCTGGGTATCA
GGCAACCTACCGTTGCAGGAATGGAAAAACCGGGCCGCGACCTGAAGCTTTCCACGCTCAAACGCTACGTTGAGGCGACC
GGCGGCAAGCTACGCCTTGATGTTGAACTGCCGGATGGTTCTCACTACGGATTTGTACTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References