Detailed information of TA system TA172642

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4768603..4769413
• Replicon: chromosome
• Accession: NZ_CP061404
• Organism: Klebsiella pneumoniae strain NU-CRE055

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: -
• Locus tag: HCV81_RS23695
• Protein ID: WP_059065756.1
• Coordinates: 4768603..4769136 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: J2E9Q7
• Locus tag: HCV81_RS23700
• Protein ID: WP_002887278.1
• Coordinates: 4769147..4769413 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV81_RS23690	4767434..4768555	+	1122	WP_002887282.1	cupin domain-containing protein	-
HCV81_RS23695	4768603..4769136	-	534	WP_059065756.1	GNAT family N-acetyltransferase	Toxin
HCV81_RS23700	4769147..4769413	-	267	WP_002887278.1	DUF1778 domain-containing protein	Antitoxin
HCV81_RS23705	4769516..4770949	-	1434	WP_048999684.1	Cu(+)/Ag(+) sensor histidine kinase	-
HCV81_RS23710	4770939..4771622	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
HCV81_RS23715	4771794..4773179	+	1386	WP_048999682.1	efflux transporter outer membrane subunit	-
HCV81_RS23720	4773197..4773541	+	345	WP_048999680.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (91-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19809.71 Da
• Isoelectric Point: 6.2369

>T172642 WP_059065756.1 NZ_CP061404:c4769136-4768603 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEKALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T172642 NZ_CP079837:c3276647-3276396 [Salmonella enterica subsp. salamae]
ATGCGTACAATTAGTTACAGCGAAGCCCGACAAAACCTGTCTGCGACAATGGTCAAAGCGGTTGAAGACCATGCGCCTAT
TCTTATTACCCGGCAAAATGGCGAGGCCTGCGTTCTTATGTCATTGGAGGAGTATAATTCTCTGGAAGAAACCGCCTATC
TTCTACGTTCCCCGGCGAATGCAAGAAGGCTGATGGACTCAATCGAAAGCCTGAAGTCAGGACGGGGCGTTGAGAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9996.46 Da
• Isoelectric Point: 10.1757

>AT172642 WP_002887278.1 NZ_CP061404:c4769413-4769147 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT172642 NZ_CP079837:c3276399-3276145 [Salmonella enterica subsp. salamae]
GTGAAACTCACCTGGTCTGAAGAAGCATGGGAGGATTATCTGTACTGGCAGGAAACAGATAAGCGTATAGTAAAAAAAAT
CAATGAATTAATTAAAGATATCCGCAGAACGCCTTTTGAAGGCAAAGGGAAACCTGAGCCGTTAAAATACAATCTCTCCG
GATTTTGGTCACGGCGTATAACAGAAGAGCATCGTCTGGTCTATGCGTTAACCGACGATGCGCTTCTTATTGCAGCCTGC
CGATATCATTATTGA

Structures

Antitoxin

• PDB: 5ZGN
• AlphaFold DB: A0A0H3GLZ1