Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 46711..47354 Replicon plasmid pCRE-085-2
Accession NZ_CP061401
Organism Klebsiella pneumoniae strain NU-CRE085

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HCV82_RS27920 Protein ID WP_001044770.1
Coordinates 46938..47354 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HCV82_RS27915 Protein ID WP_001261282.1
Coordinates 46711..46941 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV82_RS27880 42428..43183 - 756 WP_012539983.1 replication initiation protein RepE -
HCV82_RS27885 43814..43927 + 114 WP_032072094.1 hypothetical protein -
HCV82_RS27890 44056..44313 + 258 WP_032408936.1 hypothetical protein -
HCV82_RS27895 44371..45156 - 786 WP_022644886.1 site-specific integrase -
HCV82_RS27900 45159..45797 - 639 WP_022644887.1 hypothetical protein -
HCV82_RS27905 45846..46166 - 321 WP_032072060.1 hypothetical protein -
HCV82_RS27910 46294..46754 - 461 Protein_55 hypothetical protein -
HCV82_RS27915 46711..46941 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HCV82_RS27920 46938..47354 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HCV82_RS27925 47428..47664 + 237 WP_001348075.1 AAA family ATPase -
HCV82_RS27930 47711..47869 + 159 Protein_59 IS6 family transposase -
HCV82_RS27935 48036..49235 - 1200 WP_000948429.1 IS91 family transposase -
HCV82_RS27940 49245..49433 - 189 WP_000957857.1 hypothetical protein -
HCV82_RS27945 49549..50103 + 555 Protein_62 IS6-like element IS26 family transposase -
HCV82_RS27950 50156..50923 - 768 Protein_63 class 1 integron integrase IntI1 -
HCV82_RS27955 51079..51552 + 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
HCV82_RS27960 51773..52039 + 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-3 / dfrA14 / sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1A / blaOXA-9 / ant(3'')-Ia / aac(6')-Ib - 1..77139 77139


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T172628 WP_001044770.1 NZ_CP061401:46938-47354 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T172628 NZ_CP079836:190526-190678 [Salmonella enterica subsp. salamae]
ATGCCGCAGAAAGGTATGTTATACAGTTTAATAGTGATATGTCTCACTATTTTATTATTTACCTGGATAGTTCGTGATTC
ACTGTGTGAATTACATATCCGCCAGGGAAATAATGAGCTGGCGGCATCTTTAGCCTGTGATAGCAAACAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT172628 WP_001261282.1 NZ_CP061401:46711-46941 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT172628 NZ_CP079836:c190475-190420 [Salmonella enterica subsp. salamae]
GTTCAAGCATAGAGGTGGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References