Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 22701..23437 Replicon plasmid pCRE-193-1
Accession NZ_CP061397
Organism Klebsiella pneumoniae strain NU-CRE193

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag HCV83_RS27295 Protein ID WP_003026803.1
Coordinates 22955..23437 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag HCV83_RS27290 Protein ID WP_003026799.1
Coordinates 22701..22967 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV83_RS27260 19017..19697 - 681 WP_001572351.1 copper/silver response regulator transcription factor SilR -
HCV83_RS27265 19887..20531 + 645 Protein_16 Cu(+)/Ag(+) efflux RND transporter outer membrane channel SilC -
HCV83_RS27270 20601..21581 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
HCV83_RS27275 21695..21889 + 195 WP_052536218.1 hemolysin expression modulator Hha -
HCV83_RS27280 21938..22195 + 258 WP_004152107.1 hypothetical protein -
HCV83_RS27285 22271..22525 + 255 WP_004152108.1 hypothetical protein -
HCV83_RS27290 22701..22967 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
HCV83_RS27295 22955..23437 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
HCV83_RS27300 23649..24995 + 1347 WP_020314316.1 ISNCY family transposase -
HCV83_RS27305 25496..26750 - 1255 Protein_24 IS3 family transposase -
HCV83_RS27310 26838..27800 - 963 WP_004152113.1 M48 family metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aph(3')-Ia / mph(A) / sul1 / qacE / aadA2 / dfrA12 / catA1 - 1..177258 177258
- inside IScluster/Tn - - 13343..26523 13180


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T172608 WP_003026803.1 NZ_CP061397:22955-23437 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T172608 NZ_CP079808:c14256-13906 [Klebsiella pneumoniae subsp. pneumoniae]
ATGTGGGTCATCGAGACAACTGACACCTTTGATGAGTGGTTCGATGCACTGGATGATACCGATAGAGCCAACGTGCTGGC
TTCGATGATGGTGCTGCGAGATAGAGGTCCCATGCTGTCGAGACCATACGCGGATACTGTTAACGGTTCATCCTACAGCA
ACATGAAAGAGCTGCGGGTCCAAAGCAAAGGAGATCCTATCAGGGCATTCTTTGCGTTCGATCCAAAGCGTAAGGGGATT
CTTCTCTGCGCCGGTAACAAGACCGGGGACGAGAAAAGGTTTTATGAAGTAATGATCCCAATTGCAGACCGCGAGTTTGC
GGCGCACTTGGATAAATTGAAGAAGGAGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT172608 WP_003026799.1 NZ_CP061397:22701-22967 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT172608 NZ_CP079808:c13901-13599 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCAAGAACTCTTGACCAAATGCTGGCAACAGAAAAGCCTGAGGTTGTTGCCAAAGCACAGAAAGCGGCCACTGAGAT
GTTACTGAACATCCACTTGGCAGAGCTTCGTGATCGCATGAACCTTACCCAGGGGGAAATTGCTGCATCTCTGGGTGTGA
GACAGCCGACAGTCTCCGAGATGGAAAAACCTGGACGAGATCTCAAGCTGTCGTCCATTAAGCGGTACGTTGAGGCCTCC
GGTGGCAAGCTACGCCTCGATGTTGAACTTCCGGACGGCACCCACTACGGCTTTGCCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References