Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5369109..5369734 Replicon chromosome
Accession NZ_CP061389
Organism Klebsiella pneumoniae strain NU-CRE195

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HCV84_RS26145 Protein ID WP_002882817.1
Coordinates 5369109..5369492 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HCV84_RS26150 Protein ID WP_004150355.1
Coordinates 5369492..5369734 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV84_RS26130 5366475..5367377 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HCV84_RS26135 5367374..5368009 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HCV84_RS26140 5368006..5368935 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HCV84_RS26145 5369109..5369492 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HCV84_RS26150 5369492..5369734 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HCV84_RS26155 5369939..5370856 + 918 WP_004181612.1 alpha/beta hydrolase -
HCV84_RS26160 5370870..5371811 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HCV84_RS26165 5371856..5372293 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HCV84_RS26170 5372290..5373150 - 861 WP_004146232.1 virulence factor BrkB family protein -
HCV84_RS26175 5373144..5373743 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T172588 WP_002882817.1 NZ_CP061389:c5369492-5369109 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172588 NZ_CP079802:c1264007-1263639 [Klebsiella variicola]
ATACAATCCCTGCCCCCTCAAAGGGCGGCATCGTCACGTCTGTCATCAGTCGAAATCTGGCAAAAATTACTGGAGTATCT
GCTGGAGCAACACTACGGCCTTACCCTCAACGACACGCCGTTTGGCAACGACAGTGTGATCCAGAAACACATTGATGCGG
GAATCTCACTGTGTGACGCTGTAAATTTTATCGTGGAAAAGTACGACCTGGTGCGTACTGACCGTCACGGCTTCAGCGTG
AAAGAGCAGTCTCCGTTTATCGGCAGCCTTGATATGCTCCGAGCCCGGAAGGCTACCGGGCTGATGACCCGCAAGGGATA
TAAAACCGTTACTGACATCACAGGTGGCAGATTCAGCGGAGGGAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172588 WP_004150355.1 NZ_CP061389:c5369734-5369492 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172588 NZ_CP079802:c1264422-1264063 [Klebsiella variicola]
ATGAAGAAAGCAACCCGTATAATCAACCATAACATCGCGGAACCCTGGTGGGGCCTCAGACGCAACATCACGCCGTGTTT
GGGTGCCCGGCTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCTGACCGCGCCAGCATTGCCGGGACATTTAATGACA
CAGATTTACGCCATCTGGACCAGGCATTTCCGGTACTGATGAAGCAGATGGAGCTTATGCTGGCCAGCAGCGAACTCACG
CCCCACATTCAGCGCTGCATCACGCTCCACGCAAAAGGGCTGATATGTGAGGCTGATACGCTCGGCTCCTGTGGCTATTT
GTATATCGTAATTTATCCGACATCTGCAATAACCGCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References