Detailed information of TA system TA172573

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 789988..790645
• Replicon: chromosome
• Accession: NZ_CP061389
• Organism: Klebsiella pneumoniae strain NU-CRE195

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: HCV84_RS03905
• Protein ID: WP_004181233.1
• Coordinates: 790235..790645 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: W8UQ37
• Locus tag: HCV84_RS03900
• Protein ID: WP_002916312.1
• Coordinates: 789988..790254 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV84_RS03875	785144..786577	-	1434	WP_004181234.1	6-phospho-beta-glucosidase	-
HCV84_RS03880	786696..787424	-	729	WP_002916321.1	MurR/RpiR family transcriptional regulator	-
HCV84_RS03885	787474..787785	+	312	WP_004144734.1	N(4)-acetylcytidine aminohydrolase	-
HCV84_RS03890	787949..788608	+	660	WP_002916317.1	hemolysin III family protein	-
HCV84_RS03895	788759..789742	-	984	WP_002916313.1	tRNA-modifying protein YgfZ	-
HCV84_RS03900	789988..790254	+	267	WP_002916312.1	FAD assembly factor SdhE	Antitoxin
HCV84_RS03905	790235..790645	+	411	WP_004181233.1	protein YgfX	Toxin
HCV84_RS03910	790652..791173	-	522	WP_004144730.1	flavodoxin FldB	-
HCV84_RS03915	791274..792170	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
HCV84_RS03920	792193..792906	+	714	WP_002916301.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
HCV84_RS03925	792912..794645	+	1734	WP_004149758.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16121.92 Da
• Isoelectric Point: 11.1565

>T172573 WP_004181233.1 NZ_CP061389:790235-790645 [Klebsiella pneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
EWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAEEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T172573 NZ_CP079784:214456-214740 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACAATTTAACCAAACACAGAAATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10480.98 Da
• Isoelectric Point: 5.1116

>AT172573 WP_002916312.1 NZ_CP061389:789988-790254 [Klebsiella pneumoniae]
MDINNKARIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT172573 NZ_CP079784:214218-214466 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCTGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGCTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GY41