Detailed information of TA system TA172571

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 17449..18092
• Replicon: plasmid pCRE-213-2
• Accession: NZ_CP061387
• Organism: Klebsiella pneumoniae strain NU-CRE213

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: HCV85_RS28080
• Protein ID: WP_001044770.1
• Coordinates: 17676..18092 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: HCV85_RS28075
• Protein ID: WP_001261282.1
• Coordinates: 17449..17679 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV85_RS28035	12647..13120	+	474	WP_004152341.1	YkgJ family cysteine cluster protein	-
HCV85_RS28040	13212..13442	+	231	WP_011977773.1	hypothetical protein	-
HCV85_RS28045	14334..15116	-	783	WP_004152340.1	site-specific integrase	-
HCV85_RS28050	15116..15448	-	333	WP_004152339.1	hypothetical protein	-
HCV85_RS28055	15455..15853	-	399	WP_004171440.1	hypothetical protein	-
HCV85_RS28060	15879..16208	-	330	WP_004152337.1	hypothetical protein	-
HCV85_RS28065	16236..16544	-	309	WP_004152336.1	hypothetical protein	-
HCV85_RS28070	17031..17492	-	462	WP_072093212.1	hypothetical protein	-
HCV85_RS28075	17449..17679	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
HCV85_RS28080	17676..18092	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
HCV85_RS28085	18166..18876	+	711	WP_004152334.1	ATP-binding protein	-
HCV85_RS28090	18910..19607	+	698	Protein_20	IS1 family transposase	-
HCV85_RS28095	19608..19733	+	126	WP_072093211.1	mercury transporter	-
HCV85_RS28100	19769..20191	+	423	WP_001340589.1	organomercurial transporter MerC	-
HCV85_RS28105	20243..21937	+	1695	WP_000105636.1	mercury(II) reductase	-
HCV85_RS28110	21955..22317	+	363	WP_001277456.1	mercury resistance co-regulator MerD	-
HCV85_RS28115	22314..22550	+	237	WP_000993386.1	broad-spectrum mercury transporter MerE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1A / blaOXA-9 / blaKPC-3	-	1..114416	114416
	-	flank	IS/Tn	-	-	11259..12527	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T172571 WP_001044770.1 NZ_CP061387:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T172571 NZ_CP079783:32506-32811 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT172571 WP_001261282.1 NZ_CP061387:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT172571 NZ_CP079783:32286-32504 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3