Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5347917..5348542 Replicon chromosome
Accession NZ_CP061385
Organism Klebsiella pneumoniae strain NU-CRE213

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HCV85_RS26530 Protein ID WP_002882817.1
Coordinates 5347917..5348300 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HCV85_RS26535 Protein ID WP_004150355.1
Coordinates 5348300..5348542 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV85_RS26515 5345283..5346185 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HCV85_RS26520 5346182..5346817 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HCV85_RS26525 5346814..5347743 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HCV85_RS26530 5347917..5348300 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HCV85_RS26535 5348300..5348542 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HCV85_RS26540 5348747..5349664 + 918 WP_002882812.1 alpha/beta hydrolase -
HCV85_RS26545 5349678..5350619 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HCV85_RS26550 5350664..5351101 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HCV85_RS26555 5351098..5351958 - 861 WP_002882807.1 virulence factor BrkB family protein -
HCV85_RS26560 5351952..5352551 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T172569 WP_002882817.1 NZ_CP061385:c5348300-5347917 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172569 NZ_CP079782:24133-24549 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172569 WP_004150355.1 NZ_CP061385:c5348542-5348300 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172569 NZ_CP079782:23906-24136 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References