Detailed information of TA system TA172568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 5023691..5025372
• Replicon: chromosome
• Accession: NZ_CP061385
• Organism: Klebsiella pneumoniae strain NU-CRE213

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: W8V1Q6
• Locus tag: HCV85_RS25025
• Protein ID: WP_002885155.1
• Coordinates: 5023691..5024608 (-)
• Length: 306 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: W8UPQ8
• Locus tag: HCV85_RS25030
• Protein ID: WP_004151732.1
• Coordinates: 5024614..5025372 (-)
• Length: 253 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV85_RS25005	5018997..5020517	+	1521	WP_002885173.1	sugar ABC transporter ATP-binding protein	-
HCV85_RS25010	5020514..5021512	+	999	WP_002885172.1	ribose ABC transporter permease	-
HCV85_RS25015	5021543..5022484	+	942	WP_002885156.1	ABC transporter substrate-binding protein	-
HCV85_RS25020	5022559..5023566	+	1008	WP_004151731.1	carbohydrate kinase family protein	-
HCV85_RS25025	5023691..5024608	-	918	WP_002885155.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
HCV85_RS25030	5024614..5025372	-	759	WP_004151732.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
HCV85_RS25035	5025458..5026186	-	729	WP_002885152.1	response regulator transcription factor	-
HCV85_RS25040	5026459..5028606	+	2148	WP_077598858.1	formate dehydrogenase subunit alpha	-
HCV85_RS25045	5028836..5029522	+	687	WP_004151733.1	sel1 repeat family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-278)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-246)

Sequences

Toxin

Protein

• Length: 306 a.a.
• Molecular weight: 34687.15 Da
• Isoelectric Point: 5.4707

>T172568 WP_002885155.1 NZ_CP061385:c5024608-5023691 [Klebsiella pneumoniae]
MDIQAPYYRQVALLMQVLPYVAVEREFALKGGTAINLFIRDFPRLSVDIDLAWVPLESRAIALPHIRDALARIAANLQQQ
AGMSAVLQANRSDEMRVIVTTDSAQIKIEVSPVARGTLYPPQEREVVGAVEDVFGYASLPVVSLPDLYGGKLCAALDRQH
PRDFYDVKLLLDAQELDRPIFNGFIVYLLSHNRPLAEVLNPRWKDIAEPFYREFSGMTFETIALEELTAVPNRMIAALKS
CFTQQDVDFLLSFKRGEPDWRLAPEMRIQDLPAVQWKLRNIHQMPAIKRAESLDKLEKVLAEWRS

Nucleotide

• Length: 918 bp

>T172568 NZ_CP079782:c23086-22781 [Klebsiella pneumoniae subsp. pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 253 a.a.
• Molecular weight: 28564.92 Da
• Isoelectric Point: 10.0870

>AT172568 WP_004151732.1 NZ_CP061385:c5025372-5024614 [Klebsiella pneumoniae]
MATKLNWLLQNSAPGDVILQSWLSQHAISPSLSFKYAQSGWLKKRGNGVYARAGREPEWNDALACLQNQLAAPVYVAGLS
SLVWQGRSHYLQLKQNQCWLSVENKALLPKWFREFPGVEWIVISGKKLPVLDDRYRVTLDIKGKKLIGSAPELAAYELLS
AVPGTLSFNHAAELFQGLVNLNPRKVEYLLSVSQSVQAKRLYLFFASFYEHGWLKRIDSQKIDLGAGKRQIVENGKFNAQ
YQITVPERFQKE

Nucleotide

• Length: 759 bp

>AT172568 NZ_CP079782:c23306-23088 [Klebsiella pneumoniae subsp. pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GH11

Antitoxin

• AlphaFold DB: A0A0H3GQB5