Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4154815..4155434 Replicon chromosome
Accession NZ_CP061385
Organism Klebsiella pneumoniae strain NU-CRE213

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HCV85_RS20810 Protein ID WP_002892050.1
Coordinates 4155216..4155434 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HCV85_RS20805 Protein ID WP_002892066.1
Coordinates 4154815..4155189 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV85_RS20795 4149967..4151160 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HCV85_RS20800 4151183..4154329 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HCV85_RS20805 4154815..4155189 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HCV85_RS20810 4155216..4155434 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HCV85_RS20815 4155593..4156159 + 567 WP_002892030.1 maltose O-acetyltransferase -
HCV85_RS20820 4156131..4156271 - 141 WP_004147370.1 hypothetical protein -
HCV85_RS20825 4156292..4156762 + 471 WP_002892026.1 YlaC family protein -
HCV85_RS20830 4156737..4158188 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HCV85_RS20835 4158289..4158987 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
HCV85_RS20840 4158984..4159124 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HCV85_RS20845 4159124..4159387 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172565 WP_002892050.1 NZ_CP061385:4155216-4155434 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T172565 NZ_CP079781:c4706058-4705774 [Klebsiella pneumoniae subsp. pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT172565 WP_002892066.1 NZ_CP061385:4154815-4155189 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT172565 NZ_CP079781:c4706290-4706048 [Klebsiella pneumoniae subsp. pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References