Detailed information of TA system TA172556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 3299959..3301547
• Replicon: chromosome
• Accession: NZ_CP061385
• Organism: Klebsiella pneumoniae strain NU-CRE213

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: HCV85_RS16315
• Protein ID: WP_002904792.1
• Coordinates: 3299959..3301281 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: HCV85_RS16320
• Protein ID: WP_002904790.1
• Coordinates: 3301281..3301547 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV85_RS16300	3295015..3297516	+	2502	WP_004151634.1	fimbrial biogenesis outer membrane usher protein	-
HCV85_RS16305	3297504..3298508	+	1005	WP_167578749.1	type 1 fimbrial protein	-
HCV85_RS16310	3298650..3299942	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
HCV85_RS16315	3299959..3301281	-	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
HCV85_RS16320	3301281..3301547	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HCV85_RS16325	3301813..3302139	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
HCV85_RS16330	3302136..3302636	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
HCV85_RS16335	3302614..3303993	-	1380	WP_002904785.1	purine permease	-
HCV85_RS16340	3304339..3305493	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
HCV85_RS16345	3305474..3306397	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T172556 WP_002904792.1 NZ_CP061385:c3301281-3299959 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T172556 NZ_CP079780:c4113515-4113297 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT172556 WP_002904790.1 NZ_CP061385:c3301547-3301281 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT172556 NZ_CP079780:c4113915-4113541 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8

Antitoxin

• AlphaFold DB: A0A0H3GXD8