Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 68319..68962 Replicon plasmid pCRE-236-2
Accession NZ_CP061382
Organism Klebsiella pneumoniae strain NU-CRE236

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag HCV86_RS26490 Protein ID WP_001044770.1
Coordinates 68319..68735 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag HCV86_RS26495 Protein ID WP_001261282.1
Coordinates 68732..68962 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV86_RS26455 64210..64275 + 66 Protein_68 EAL domain-containing protein -
HCV86_RS26460 64415..65179 - 765 WP_001389365.1 IS6-like element IS6100 family transposase -
HCV86_RS26465 65322..65588 - 267 WP_001144737.1 plasmid mobilization relaxosome protein MobC -
HCV86_RS26470 65809..66282 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
HCV86_RS26475 66438..67205 + 768 Protein_72 class 1 integron integrase IntI1 -
HCV86_RS26480 67258..67962 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
HCV86_RS26485 68009..68245 - 237 WP_001348075.1 AAA family ATPase -
HCV86_RS26490 68319..68735 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
HCV86_RS26495 68732..68962 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HCV86_RS26500 68919..69379 + 461 Protein_77 hypothetical protein -
HCV86_RS26505 69507..69827 + 321 WP_032072060.1 hypothetical protein -
HCV86_RS26510 69876..70514 + 639 WP_022644887.1 hypothetical protein -
HCV86_RS26515 70517..71302 + 786 WP_022644886.1 site-specific integrase -
HCV86_RS26520 71360..71617 - 258 WP_032408936.1 hypothetical protein -
HCV86_RS26525 71746..71859 - 114 WP_032072094.1 hypothetical protein -
HCV86_RS26530 72490..73245 + 756 WP_012539983.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-3 / dfrA14 - 1..136387 136387


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T172551 WP_001044770.1 NZ_CP061382:c68735-68319 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T172551 NZ_CP079780:3385184-3385357 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT172551 WP_001261282.1 NZ_CP061382:c68962-68732 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT172551 NZ_CP079780:3384860-3385156 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References