172550

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 84924..85446 Replicon plasmid pCRE-236-1
Accession NZ_CP061381
Organism Klebsiella pneumoniae strain NU-CRE236

Toxin (Protein)


Gene name stbE Uniprot ID A0A9J6S4Z8
Locus tag HCV86_RS25750 Protein ID WP_004187019.1
Coordinates 85162..85446 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4ZSR4
Locus tag HCV86_RS25745 Protein ID WP_004187025.1
Coordinates 84924..85172 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV86_RS25715 82291..82860 - 570 WP_004152117.1 small heat shock protein sHSP20 -
HCV86_RS25720 82895..83176 - 282 WP_013307885.1 helix-turn-helix domain-containing protein -
HCV86_RS25725 83420..83683 - 264 WP_004118208.1 hypothetical protein -
HCV86_RS25730 83698..83961 + 264 WP_004187027.1 transposase -
HCV86_RS25735 83906..84022 + 117 Protein_83 transposase domain-containing protein -
HCV86_RS25740 84210..84634 + 425 Protein_84 IS1 family transposase -
HCV86_RS25745 84924..85172 + 249 WP_004187025.1 plasmid stabilization protein Antitoxin
HCV86_RS25750 85162..85446 + 285 WP_004187019.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HCV86_RS25755 85462..86817 - 1356 WP_004187017.1 ISNCY family transposase -
HCV86_RS25760 86899..87198 - 300 Protein_88 conjugal transfer protein TraH -
HCV86_RS25765 87196..87679 - 484 Protein_89 hypothetical protein -
HCV86_RS25770 87696..88220 - 525 Protein_90 hypothetical protein -
HCV86_RS25775 89763..90227 + 465 WP_004187010.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - mrkA / mrkB / mrkC / mrkF / mrkJ 1..161774 161774
- flank IS/Tn - - 84404..84670 266


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 11003.72 Da        Isoelectric Point: 10.5388

>T172550 WP_004187019.1 NZ_CP061381:85162-85446 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLRGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Download         Length: 285 bp

>T172550 NZ_CP079780:c2725732-2725625 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9011.24 Da        Isoelectric Point: 3.9947

>AT172550 WP_004187025.1 NZ_CP061381:84924-85172 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGEKVIEVNID
DL

Download         Length: 249 bp

>AT172550 NZ_CP079780:2725780-2725846 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZSR4

References