Detailed information of TA system TA172549

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 71293..72029
• Replicon: plasmid pCRE-236-1
• Accession: NZ_CP061381
• Organism: Klebsiella pneumoniae strain NU-CRE236

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: HCV86_RS25660
• Protein ID: WP_004187044.1
• Coordinates: 71293..71775 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HCV86_RS25665
• Protein ID: WP_003026799.1
• Coordinates: 71763..72029 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HCV86_RS25635	67318..68254	-	937	Protein_63	ISNCY family transposase	-
HCV86_RS25640	68364..68534	+	171	Protein_64	transposase	-
HCV86_RS25645	68532..68639	+	108	Protein_65	IS3 family transposase	-
HCV86_RS25650	68872..69576	-	705	WP_031591821.1	toll/interleukin-1 receptor domain-containing protein	-
HCV86_RS25655	69736..71082	-	1347	WP_077251107.1	ISNCY family transposase	-
HCV86_RS25660	71293..71775	-	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
HCV86_RS25665	71763..72029	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HCV86_RS25670	72180..72884	+	705	WP_060579418.1	IS6-like element IS26 family transposase	-
HCV86_RS25675	73043..75628	-	2586	WP_167580763.1	EAL domain-containing protein	-
HCV86_RS25680	75597..75842	-	246	WP_032238678.1	hypothetical protein	-
HCV86_RS25685	75900..76880	-	981	Protein_73	IS5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	mrkA / mrkB / mrkC / mrkF / mrkJ	1..161774	161774
	-	inside	IScluster/Tn	-	-	67297..76880	9583

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T172549 WP_004187044.1 NZ_CP061381:c71775-71293 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T172549 NZ_CP079780:2299346-2299810 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT172549 WP_003026799.1 NZ_CP061381:c72029-71763 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT172549 NZ_CP079780:2299011-2299346 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66