172548

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5132269..5132894 Replicon chromosome
Accession NZ_CP061380
Organism Klebsiella pneumoniae strain NU-CRE236

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag HCV86_RS24955 Protein ID WP_004187928.1
Coordinates 5132269..5132652 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HCV86_RS24960 Protein ID WP_004150355.1
Coordinates 5132652..5132894 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV86_RS24940 5129635..5130537 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HCV86_RS24945 5130534..5131169 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HCV86_RS24950 5131166..5132095 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HCV86_RS24955 5132269..5132652 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
HCV86_RS24960 5132652..5132894 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
HCV86_RS24965 5133088..5134005 + 918 WP_004187929.1 alpha/beta hydrolase -
HCV86_RS24970 5134019..5134960 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HCV86_RS24975 5135005..5135442 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
HCV86_RS24980 5135439..5136299 - 861 WP_004146232.1 virulence factor BrkB family protein -
HCV86_RS24985 5136293..5136892 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T172548 WP_004187928.1 NZ_CP061380:c5132652-5132269 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172548 NZ_CP079780:c2256486-2256172 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172548 WP_004150355.1 NZ_CP061380:c5132894-5132652 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172548 NZ_CP079780:c2256175-2255759 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References