Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3933733..3934352 Replicon chromosome
Accession NZ_CP061380
Organism Klebsiella pneumoniae strain NU-CRE236

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag HCV86_RS19265 Protein ID WP_002892050.1
Coordinates 3934134..3934352 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag HCV86_RS19260 Protein ID WP_002892066.1
Coordinates 3933733..3934107 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HCV86_RS19250 3928885..3930078 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HCV86_RS19255 3930101..3933247 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
HCV86_RS19260 3933733..3934107 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
HCV86_RS19265 3934134..3934352 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
HCV86_RS19270 3934511..3935077 + 567 WP_004191639.1 maltose O-acetyltransferase -
HCV86_RS19275 3935049..3935189 - 141 WP_004147370.1 hypothetical protein -
HCV86_RS19280 3935210..3935680 + 471 WP_002892026.1 YlaC family protein -
HCV86_RS19285 3935655..3937106 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
HCV86_RS19290 3937207..3937905 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
HCV86_RS19295 3937902..3938042 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
HCV86_RS19300 3938042..3938305 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T172544 WP_002892050.1 NZ_CP061380:3934134-3934352 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T172544 NZ_CP079780:c1180242-1179991 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT172544 WP_002892066.1 NZ_CP061380:3933733-3934107 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT172544 NZ_CP079780:c1179994-1179740 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References