Detailed information of TA system TA172524

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 5243506..5244107
• Replicon: chromosome
• Accession: NZ_CP061378
• Organism: Bradyrhizobium genosp. L strain BDV5419

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: IC762_RS25010
• Protein ID: WP_195784869.1
• Coordinates: 5243506..5243796 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: IC762_RS25015
• Protein ID: WP_195784870.1
• Coordinates: 5243808..5244107 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC762_RS25000	5238755..5239432	-	678	WP_195790268.1	hypothetical protein	-
IC762_RS25005	5239775..5243446	+	3672	WP_195784868.1	autotransporter domain-containing protein	-
IC762_RS25010	5243506..5243796	+	291	WP_195784869.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC762_RS25015	5243808..5244107	+	300	WP_195784870.1	putative addiction module antidote protein	Antitoxin
IC762_RS25020	5244128..5244691	-	564	WP_195784871.1	type II secretion system protein M	-
IC762_RS25025	5244688..5245752	-	1065	WP_195784872.1	PilN domain-containing protein	-
IC762_RS25030	5245749..5246741	-	993	WP_195784873.1	general secretion pathway protein GspK	-
IC762_RS25035	5246660..5247385	-	726	WP_195784874.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
IC762_RS25040	5247382..5247840	-	459	WP_195790269.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
IC762_RS25045	5247863..5248399	-	537	WP_195784875.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
IC762_RS25050	5248320..5248778	-	459	WP_195784876.1	type II secretion system major pseudopilin GspG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Uncharacterized protein HI1420 (3-92)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10728.35 Da
• Isoelectric Point: 9.8176

>T172524 WP_195784869.1 NZ_CP061378:5243506-5243796 [Bradyrhizobium genosp. L]
MFEVLKTDLFDSWLSNLTDRRAAAKIASRIERLALGNPGDVKPVGEGVSEMRLAYGPGYRVYYKQTGQRIILILCGGDKS
NQDRDIKRAKEIAAQL

Nucleotide

• Length: 291 bp

>T172524 NZ_CP079775:18805-19056 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10846.55 Da
• Isoelectric Point: 5.1598

>AT172524 WP_195784870.1 NZ_CP061378:5243808-5244107 [Bradyrhizobium genosp. L]
MVKISKFDAADYLKDPVSIAAYLTEAFETDDPVYISMALDTVARAKGIADIAKATGLSRESLYKTFKETARPEFETVRKV
MSSLGVKLVVEPIDAQKVA

Nucleotide

• Length: 300 bp

>AT172524 NZ_CP079775:19043-19270 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA