Detailed information of TA system TA172523

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 4803683..4804205
• Replicon: chromosome
• Accession: NZ_CP061378
• Organism: Bradyrhizobium genosp. L strain BDV5419

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: IC762_RS22865
• Protein ID: WP_195784473.1
• Coordinates: 4803683..4803943 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: IC762_RS22870
• Protein ID: WP_195784474.1
• Coordinates: 4803924..4804205 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC762_RS22845	4799563..4800714	-	1152	WP_195790244.1	zinc-binding dehydrogenase	-
IC762_RS22850	4800871..4801839	+	969	WP_210338387.1	acyl-CoA desaturase	-
IC762_RS22855	4801967..4802584	+	618	WP_195784471.1	PilZ domain-containing protein	-
IC762_RS22860	4802918..4803532	+	615	WP_195784472.1	transglutaminase-like cysteine peptidase	-
IC762_RS22865	4803683..4803943	+	261	WP_195784473.1	BrnT family toxin	Toxin
IC762_RS22870	4803924..4804205	+	282	WP_195784474.1	BrnA antitoxin family protein	Antitoxin
IC762_RS22875	4804468..4807662	-	3195	WP_195784475.1	efflux RND transporter permease subunit	-
IC762_RS22880	4807946..4808356	-	411	WP_195784476.1	hypothetical protein	-
IC762_RS22885	4808456..4809070	+	615	WP_195784477.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (20-87)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9797.31 Da
• Isoelectric Point: 10.8407

>T172523 WP_195784473.1 NZ_CP061378:4803683-4803943 [Bradyrhizobium genosp. L]
MKIIWDEPKRLANLAKHGMDFADLNETFFDTALAVATYRNRWRAIGVNIRGVISVIFATRGTEAISIVSMRPASKNERAL
YEANRR

Nucleotide

• Length: 261 bp

>T172523 NZ_CP079773:35466-35987 [Escherichia coli]
GTGAGCAATACGACGATCGAGATTTTCTCTGGAGAGAAAGATTATGATCTAAACGGTTTTGATTGCGGCGAAGAGTCGCT
AAACGCATTTTTGACCAACCACTTAAAAAGACAGCATGAGGGAAAAATTCTTCGCGCTTATGTGCTTTGCACTAAAGAAG
AAATACCAAAGGTATTAGGTTATTACACTTTGTCAGGCAGCTGCTTTGAGAAAGAAACCTTACCTTCAAAGAGTCAGCAA
AAGAAAGTGCCTTATCGGAATGTCCCAAGTATCACTTTAGGCAGACTGGCTTTGGACAAATCTCTTCAAGGTCAGGGGCT
TGGCTCAATGCTTGTAACTCACGCAATGCGTGTTGTGTACAATGCATCTCTTGCGGTAGGCATTCATGGGCTTTTCGTTG
AGGCGTTGAACGATAAAGCCAGGGCGTTTTATAAAAGTTTGGGCTTTATCCAGCTGGTTGGTAACAACGATCGTTCTTTG
TTCTATCCAACAAAATCTATCGAAAAATTGTTCGAAGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10456.23 Da
• Isoelectric Point: 11.3011

>AT172523 WP_195784474.1 NZ_CP061378:4803924-4804205 [Bradyrhizobium genosp. L]
MKRTVVKKGYTAKDVRAVSDNPEWTKQDFSKAKPFNEVFATMRKGRGPNKIPTKTQVTLRLSPAVLEHFKAAGPGWQSRI
DEALVKMVKRKAG

Nucleotide

• Length: 282 bp

>AT172523 NZ_CP079773:35189-35458 [Escherichia coli]
ATGTCCGCACTGAAAAAGCAGCGCATCGATTTGAGATTAACCGATGACGATAAAAGCATCATCGAGGAAGCTGCCGCAAT
GTCTAACCAGAGCATTACCCAGTTCATGGTTAGCAGTGCATCCGAACGTGCCGTGAAGGTGATAGAGCAACACCGTAGAC
TGGTTCTAAGTGAAGAGTCCTGGAATCTGGTTATGGATGCTATAAGCAATCCTCCGGCACCGAACGACAAGCTGAAACGA
GCTGCCAATCGTCTGAAAAGCATGGAGTAA