Detailed information of TA system TA172521

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): slvAT/RES(toxin)
• Location: 36649..37565
• Replicon: plasmid pHS17-127
• Accession: NZ_CP061377
• Organism: Pseudomonas aeruginosa strain HS17-127

Toxin (Protein)

• Gene name: slvT
• Uniprot ID: -
• Locus tag: ICI41_RS29770
• Protein ID: WP_065760303.1
• Coordinates: 37041..37565 (+)
• Length: 175 a.a.

Antitoxin (Protein)

• Gene name: slvA
• Uniprot ID: A0A2L1KH13
• Locus tag: ICI41_RS29765
• Protein ID: WP_019437782.1
• Coordinates: 36649..37044 (+)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI41_RS29750 (ICI41_29745)	32290..32814	-	525	WP_010794447.1	hypothetical protein	-
ICI41_RS29755 (ICI41_29750)	33681..33962	-	282	WP_065760301.1	hypothetical protein	-
ICI41_RS29760 (ICI41_29755)	34162..36045	+	1884	WP_223218642.1	helicase	-
ICI41_RS29765 (ICI41_29760)	36649..37044	+	396	WP_019437782.1	hypothetical protein	Antitoxin
ICI41_RS29770 (ICI41_29765)	37041..37565	+	525	WP_065760303.1	RES family NAD+ phosphorylase	Toxin
ICI41_RS29775 (ICI41_29770)	37611..38861	-	1251	WP_065760304.1	TerD family protein	-
ICI41_RS29780 (ICI41_29775)	39166..39357	+	192	WP_033997860.1	hypothetical protein	-
ICI41_RS29785 (ICI41_29780)	39426..39698	+	273	WP_065760305.1	hypothetical protein	-
ICI41_RS29790 (ICI41_29785)	39778..40923	-	1146	WP_065760306.1	glycerate kinase	-
ICI41_RS29795 (ICI41_29790)	41048..42133	-	1086	WP_065760307.1	sugar diacid recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaIMP-45 / blaOXA-1 / catB3 / qacE / sul1 / qnrVC6 / blaPER-1 / blaNDM-14 / msr(E) / mph(E) / aph(3')-Ia / tet(C)	-	1..486963	486963

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (11-167)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 175 a.a.
• Molecular weight: 19028.50 Da
• Isoelectric Point: 4.5535

>T172521 WP_065760303.1 NZ_CP061377:37041-37565 [Pseudomonas aeruginosa]
MKIIGVQDLSCYRMHTPKWSVAPTSGAGAATQGGRVNRKGLEALYLSLESATAIAEFQQASAFLPPGLLVSYSLSLDRVI
DFTGGYNDTWAPIWQDFFCDWRKIRFNDGVEPPSWIIGDLAIEADCAGILFESVANPGGRNLVLFTDQLPVHGNIVVNDP
RGDLPTDQSSWTRP

Nucleotide

• Length: 525 bp

>T172521 NZ_CP079772:4454962-4455297 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14753.87 Da
• Isoelectric Point: 7.2048

>AT172521 WP_019437782.1 NZ_CP061377:36649-37044 [Pseudomonas aeruginosa]
MASKAVLELRPTNFAGLMDALKEVNRVMFSPERFLKVFSMDQQTLALRAHVHRNTVRNAPESEKVQAYIRDSVKVLRAVT
DMGTDVTNAIFWFKNEPLSTFGYKTAEEVVSEGKTEQLIAFLQSWEAGAQG

Nucleotide

• Length: 396 bp

>AT172521 NZ_CP079772:4454714-4454962 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2L1KH13