Detailed information of TA system TA172519

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 4465779..4467037
• Replicon: chromosome
• Accession: NZ_CP061376
• Organism: Pseudomonas aeruginosa strain HS17-127

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ICI41_RS20675
• Protein ID: WP_003163133.1
• Coordinates: 4465779..4466534 (-)
• Length: 252 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: V6AJU7
• Locus tag: ICI41_RS20680
• Protein ID: WP_003086329.1
• Coordinates: 4466669..4467037 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICI41_RS20665 (ICI41_20660)	4460951..4463494	+	2544	WP_031630217.1	acyl-homoserine-lactone acylase QuiP	-
ICI41_RS20670 (ICI41_20665)	4463649..4465010	-	1362	WP_003081943.1	DNA recombination protein RmuC	-
ICI41_RS20675 (ICI41_20670)	4465779..4466534	-	756	WP_003163133.1	RES family NAD+ phosphorylase	Toxin
ICI41_RS20680 (ICI41_20675)	4466669..4467037	-	369	WP_003086329.1	DUF2384 domain-containing protein	Antitoxin
ICI41_RS20685 (ICI41_20680)	4467110..4468396	-	1287	WP_003163134.1	FAD-binding oxidoreductase	-
ICI41_RS20690 (ICI41_20685)	4468452..4470041	-	1590	WP_003123191.1	aldehyde dehydrogenase family protein	-
ICI41_RS20695 (ICI41_20690)	4470119..4470595	+	477	WP_031675481.1	elongation factor GreAB	-
ICI41_RS20700 (ICI41_20695)	4470598..4470873	-	276	WP_169057979.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (63-207)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-47)

Sequences

Toxin

Protein

• Length: 252 a.a.
• Molecular weight: 28250.37 Da
• Isoelectric Point: 7.7294

>T172519 WP_003163133.1 NZ_CP061376:c4466534-4465779 [Pseudomonas aeruginosa]
VSEIWRQCKGERHIRPLQGRLVRLVESQEQVATLQLVDTLEEQALLEELLESSKPPVPADAEPLHYLLKTPFRYPPLRWG
SRFGRRHEPSLFYAALKLETAMAESAYYRCVLWSGMAVPPPSGRILSEHASFEAGWKVERGIRLQAPPFSDHEAALTDIA
DYRAPQELGSAMRSAGVQAFEYRSARCPERGCNVALFTPAAFTEKRPRNLTPWLCETTAGYVAFKPAHVPGSPKIFSWEL
FLVDGKLPHPA

Nucleotide

• Length: 756 bp

>T172519 NZ_CP079772:4259287-4259583 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13367.63 Da
• Isoelectric Point: 9.9874

>AT172519 WP_003086329.1 NZ_CP061376:c4467037-4466669 [Pseudomonas aeruginosa]
MSALIKERPSADAVLAKAVLAAREQLGLTQLELAGIVGVDRSAISRWKTQGLRVDSKTGELALLLVRVYRALYALFGGQQ
EDMRHFLRTPNHHLAGEPLALMGQVQGLVHVLEYLDAIRGKV

Nucleotide

• Length: 369 bp

>AT172519 NZ_CP079772:4259585-4259980 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Antitoxin

• AlphaFold DB: V6AJU7