Detailed information of TA system TA172500

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 3472170..3472702
• Replicon: chromosome
• Accession: NZ_CP061368
• Organism: Wenzhouxiangella sp. AB-CW3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IC757_RS14970
• Protein ID: WP_190975087.1
• Coordinates: 3472379..3472702 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: IC757_RS14965
• Protein ID: WP_190975086.1
• Coordinates: 3472170..3472382 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC757_RS14955	3469709..3470626	+	918	WP_190975084.1	oxygen-dependent coproporphyrinogen oxidase	-
IC757_RS14960	3470730..3471767	-	1038	WP_190975085.1	hypothetical protein	-
IC757_RS14965	3472170..3472382	+	213	WP_190975086.1	antitoxin MazE family protein	Antitoxin
IC757_RS14970	3472379..3472702	+	324	WP_190975087.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IC757_RS14975	3472808..3474016	-	1209	WP_190977058.1	amidohydrolase family protein	-
IC757_RS14980	3474028..3475332	-	1305	WP_190975088.1	amidohydrolase family protein	-
IC757_RS14985	3475583..3476602	-	1020	WP_190975089.1	serine hydrolase	-
IC757_RS14990	3476609..3477265	+	657	WP_190975090.1	DNA oxidative demethylase AlkB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-106)

Antitoxin

• Antitoxin MazE-like (6-69)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11789.85 Da
• Isoelectric Point: 10.3461

>T172500 WP_190975087.1 NZ_CP061368:3472379-3472702 [Wenzhouxiangella sp. AB-CW3]
VKRGELVTVALKGGYGKPRPALVIQSDLFDAHPSVTVMPVTSELRDTPLFRFDISPGRVNGLKHPSQVMIDKIHTVGRDR
VGPVIGRLEEEQMVSVNRLMMVFLGLA

Nucleotide

• Length: 324 bp

>T172500 NZ_CP079770:4446531-4446866 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8345.25 Da
• Isoelectric Point: 6.4565

>AT172500 WP_190975086.1 NZ_CP061368:3472170-3472382 [Wenzhouxiangella sp. AB-CW3]
MSHSVRDRVSSYRARMRRAGLRPVQIWVPDTRRPGFAEECRRQSFMLHDDRQEADLADWLDQVADDDGWQ

Nucleotide

• Length: 213 bp

>AT172500 NZ_CP079770:4446283-4446531 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA