Detailed information of TA system TA172493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 193761..195375
• Replicon: chromosome
• Accession: NZ_CP061368
• Organism: Wenzhouxiangella sp. AB-CW3

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: IC757_RS00870
• Protein ID: WP_190976889.1
• Coordinates: 194869..195375 (-)
• Length: 169 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: IC757_RS00865
• Protein ID: WP_190975531.1
• Coordinates: 193761..194852 (-)
• Length: 364 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC757_RS00850	189167..189895	-	729	WP_190975528.1	M48 family metallopeptidase	-
IC757_RS00855	189885..192965	-	3081	WP_190975529.1	type I restriction endonuclease subunit R	-
IC757_RS00860	193011..193673	-	663	WP_190975530.1	hypothetical protein	-
IC757_RS00865	193761..194852	-	1092	WP_190975531.1	macro domain-containing protein	Antitoxin
IC757_RS00870	194869..195375	-	507	WP_190976889.1	DUF4433 domain-containing protein	Toxin
IC757_RS00875	195667..197768	-	2102	Protein_169	DUF262 domain-containing protein	-
IC757_RS00880	197765..199120	-	1356	WP_190975532.1	restriction endonuclease subunit S	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (1-167)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 169 a.a.
• Molecular weight: 19131.67 Da
• Isoelectric Point: 5.2336

>T172493 WP_190976889.1 NZ_CP061368:c195375-194869 [Wenzhouxiangella sp. AB-CW3]
IQASRHDRAIPCGPGGSMHDYVPFYFGPLSPMMLNLKTGRVAGYQEGQEPLIYLVSTAQKVQETGAEFVFSDGHGLAAFT
EWFDDLDFLEQIDWQVVKARYWADTANDMDRQRKKQAEFLVHRFCPWALIGEIVVLNQRMKERVEAVLAGYPAASGVAVS
ERPDWYYH

Nucleotide

• Length: 507 bp

>T172493 NZ_CP079770:3641619-3641726 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin

Protein

• Length: 364 a.a.
• Molecular weight: 41207.87 Da
• Isoelectric Point: 6.9363

>AT172493 WP_190975531.1 NZ_CP061368:c194852-193761 [Wenzhouxiangella sp. AB-CW3]
MIEHTQGNLLSADAEALVNTVNCVGYMGKGIALQFKQAFPENFKAYQRACKAEEVRPGRMFVFETGNLVNPRYIINFPTK
NHWRGKSRMQDIEQGLQDLIAVIRRLNIRSVAIPPLGSGLGGLNWLEVKPRIEAAFADLADVHVLLYEPKGAPEAARMLV
RTKRPHWTVARALFISLMDRYGELDYRRTLLEIQKLAYFLQEAGEGLRLRYEKGLYGPYAHNLNKVLETLEGHYTRGFGD
SQKPDTEIELMDGAVDEAERFLAEYQESRERLDQVTELIAGFETPYGMELLSSVHWVAAHDERLVSNADEAVEAVHAWND
RKRSMFQPKHIRVAWRHLEDHGWLGLDQAASRGPGSGNAGRPR

Nucleotide

• Length: 1092 bp

>AT172493 NZ_CP079770:c3641571-3641505 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT