Detailed information of TA system TA172490

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 4479178..4480015
• Replicon: chromosome
• Accession: NZ_CP061367
• Organism: Shigella sonnei strain 6207

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: IC759_RS23510
• Protein ID: WP_000227791.1
• Coordinates: 4479473..4480015 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: IC759_RS23505
• Protein ID: WP_001297137.1
• Coordinates: 4479178..4479489 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC759_RS23480 (4474198)	4474198..4475145	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
IC759_RS23485 (4475167)	4475167..4477158	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
IC759_RS23490 (4477148)	4477148..4477762	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
IC759_RS23495 (4477762)	4477762..4478091	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
IC759_RS23500 (4478103)	4478103..4478993	+	891	WP_000971327.1	heme o synthase	-
IC759_RS23505 (4479178)	4479178..4479489	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
IC759_RS23510 (4479473)	4479473..4480015	+	543	WP_000227791.1	GNAT family N-acetyltransferase	Toxin
IC759_RS23515 (4480071)	4480071..4480637	-	567	Protein_4427	SEL1-like repeat protein	-
IC759_RS23520 (4480671)	4480671..4481368	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
IC759_RS23525 (4481385)	4481385..4481783	-	399	Protein_4429	SEL1-like repeat protein	-
IC759_RS23535 (4482968)	4482968..4484332	+	1365	WP_001000971.1	MFS transporter	-
IC759_RS23540 (4484460)	4484460..4484951	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19794.98 Da
• Isoelectric Point: 8.3395

>T172490 WP_000227791.1 NZ_CP061367:4479473-4480015 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNSVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T172490 NZ_CP079770:2500890-2501168 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT172490 WP_001297137.1 NZ_CP061367:4479178-4479489 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT172490 NZ_CP079770:2500627-2500887 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3