Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1686502..1687156 Replicon chromosome
Accession NZ_CP061367
Organism Shigella sonnei strain 6207

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IC759_RS09250 Protein ID WP_000244777.1
Coordinates 1686749..1687156 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IC759_RS09245 Protein ID WP_000354046.1
Coordinates 1686502..1686768 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC759_RS09220 (1681671) 1681671..1682414 + 744 WP_000951938.1 SDR family oxidoreductase -
IC759_RS09225 (1682471) 1682471..1683904 - 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
IC759_RS09230 (1683949) 1683949..1684260 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
IC759_RS09235 (1684424) 1684424..1685083 + 660 WP_000250274.1 hemolysin III family protein -
IC759_RS09240 (1685279) 1685279..1686259 - 981 WP_000886060.1 tRNA-modifying protein YgfZ -
IC759_RS09245 (1686502) 1686502..1686768 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IC759_RS09250 (1686749) 1686749..1687156 + 408 WP_000244777.1 protein YgfX Toxin
IC759_RS09255 (1687196) 1687196..1687717 - 522 WP_001055874.1 flavodoxin FldB -
IC759_RS09260 (1687829) 1687829..1688725 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IC759_RS09265 (1688750) 1688750..1689460 + 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IC759_RS09270 (1689466) 1689466..1691199 + 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T172476 WP_000244777.1 NZ_CP061367:1686749-1687156 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172476 NZ_CP079767:4228613-4228990 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAGCTTATTGCAATTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCAGGCATGTC
AGATTCGGGAAGAGCTGAGGCTATTATCGGGAGAGTCCAGGCCAGAGTTGCATATGAAGAGATTACCGGCCTTTTCGAAG
TCTCCGCAACTTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
TTGTTATTTCTGCGCCGTAACGGTGTGCAGGTATATGATTCACCAGAACTGGCAGAGCTTACTGTGGGGGCTGCGACCGG
AGAGGTATCCGTATCTACTGTCGTTGCCACGTTACGTAGATTGTATGGTACGGCGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172476 WP_000354046.1 NZ_CP061367:1686502-1686768 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172476 NZ_CP079767:4228392-4228613 [Escherichia coli]
ATGCAATCTATTAACTTCCGTACCGCACGCAGCAATCTTACCGAAGTGCTCAACAATGTTGAAGCCGGAGAAGAGGTTGA
AATCACCCGTAGAGGTCGTGAGCCAGCTGTAATTGTCAGCAAGGCCACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTCGCATCCCTGTTTGACACTCTGGACACCACCAATAAGAAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References