Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 298415..298912 Replicon chromosome
Accession NZ_CP061367
Organism Shigella sonnei strain 6207

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag IC759_RS02340 Protein ID WP_001173343.1
Coordinates 298415..298588 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag IC759_RS02345 Protein ID WP_000398619.1
Coordinates 298616..298912 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC759_RS02320 (293567) 293567..294723 + 1157 WP_087879964.1 IS3-like element IS600 family transposase -
IC759_RS02325 (294769) 294769..295026 + 258 Protein_293 lysine decarboxylase LdcC -
IC759_RS02330 (295085) 295085..296542 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
IC759_RS02335 (296779) 296779..298296 + 1518 WP_001295086.1 lysine--tRNA ligase -
IC759_RS02340 (298415) 298415..298588 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
IC759_RS02345 (298616) 298616..298912 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
IC759_RS02350 (299139) 299139..299411 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
IC759_RS02355 (299423) 299423..299653 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
IC759_RS02360 (299834) 299834..301465 + 1632 WP_001216495.1 sensor histidine kinase -
IC759_RS02365 (301462) 301462..302181 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T172473 WP_001173343.1 NZ_CP061367:c298588-298415 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T172473 NZ_CP079767:4087969-4088283 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT172473 WP_000398619.1 NZ_CP061367:c298912-298616 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT172473 NZ_CP079767:4088280-4088696 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References