Detailed information of TA system TA172470

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 36754..37279
• Replicon: plasmid pMDR
• Accession: NZ_CP061352
• Organism: Klebsiella pneumoniae strain ENT1988

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: J5VBT8
• Locus tag: ICN80_RS26540
• Protein ID: WP_004197633.1
• Coordinates: 36974..37279 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: ICN80_RS26535
• Protein ID: WP_004197642.1
• Coordinates: 36754..36972 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICN80_RS26495	32805..33128	+	324	WP_019725650.1	hypothetical protein	-
ICN80_RS26500	33531..33785	-	255	WP_065810046.1	hypothetical protein	-
ICN80_RS26505	33861..34118	-	258	WP_047665813.1	hypothetical protein	-
ICN80_RS26510	34167..34370	-	204	WP_004152106.1	hemolysin expression modulator Hha	-
ICN80_RS26515	34404..34772	-	369	WP_110921449.1	hypothetical protein	-
ICN80_RS26520	34816..35310	-	495	WP_020805591.1	hypothetical protein	-
ICN80_RS26525	35341..35913	-	573	WP_015065504.1	hypothetical protein	-
ICN80_RS26530	35910..36158	-	249	WP_004200907.1	hypothetical protein	-
ICN80_RS26535	36754..36972	+	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
ICN80_RS26540	36974..37279	+	306	WP_004197633.1	type II toxin-antitoxin system toxin CcdB	Toxin
ICN80_RS26545	37448..37849	+	402	WP_072199347.1	hypothetical protein	-
ICN80_RS26550	37876..38199	+	324	WP_004197641.1	hypothetical protein	-
ICN80_RS26555	38196..39212	+	1017	WP_004197639.1	hypothetical protein	-
ICN80_RS26560	39410..40204	+	795	WP_004197635.1	site-specific integrase	-
ICN80_RS26565	40684..40863	-	180	WP_006788217.1	hypothetical protein	-
ICN80_RS26570	40983..41609	-	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-1 / aac(6')-Ib-cr / tet(A) / qnrB1 / dfrA14	-	1..108694	108694

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11585.27 Da
• Isoelectric Point: 6.4661

>T172470 WP_004197633.1 NZ_CP061352:36974-37279 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T172470 NZ_CP079767:c3003189-3003016 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT172470 WP_004197642.1 NZ_CP061352:36754-36972 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT172470 NZ_CP079767:c3003513-3003217 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A2A5MCG5