Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 53136..53872 Replicon plasmid pKpVP
Accession NZ_CP061351
Organism Klebsiella pneumoniae strain ENT1988

Toxin (Protein)


Gene name tacT Uniprot ID A0A2J5Q928
Locus tag ICN80_RS25495 Protein ID WP_004098919.1
Coordinates 53390..53872 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7D3T1D0
Locus tag ICN80_RS25490 Protein ID WP_004213599.1
Coordinates 53136..53402 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICN80_RS25465 48368..48925 + 558 WP_004213590.1 recombinase family protein -
ICN80_RS25470 48928..51897 + 2970 WP_004213592.1 Tn3 family transposase -
ICN80_RS25475 51939..52433 + 495 WP_004213594.1 hypothetical protein -
ICN80_RS25480 52494..52697 + 204 WP_004213596.1 hemolysin expression modulator Hha -
ICN80_RS25485 52711..52941 + 231 WP_004213598.1 hypothetical protein -
ICN80_RS25490 53136..53402 + 267 WP_004213599.1 DUF1778 domain-containing protein Antitoxin
ICN80_RS25495 53390..53872 + 483 WP_004098919.1 GNAT family N-acetyltransferase Toxin
ICN80_RS25500 54293..55520 + 1228 Protein_51 IS3 family transposase -
ICN80_RS25505 55516..55911 - 396 Protein_52 DDE-type integrase/transposase/recombinase -
ICN80_RS25510 56086..57108 - 1023 WP_004214536.1 porphobilinogen synthase -
ICN80_RS25515 57172..58518 - 1347 WP_011154555.1 dihydroorotase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 48368..55520 7152
- inside Mobilizable plasmid - iroN / rmpA / iucA / iucB / iucC / iucD / iutA / rmpA2 1..200067 200067


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(66-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17335.06 Da        Isoelectric Point: 10.0704

>T172468 WP_004098919.1 NZ_CP061351:53390-53872 [Klebsiella pneumoniae]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T172468 NZ_CP079767:2495702-2495980 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10151.58 Da        Isoelectric Point: 4.5141

>AT172468 WP_004213599.1 NZ_CP061351:53136-53402 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT172468 NZ_CP079767:2495439-2495699 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J5Q928

Antitoxin

Source ID Structure
AlphaFold DB A0A7D3T1D0

References