172467

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5170216..5170841 Replicon chromosome
Accession NZ_CP061350
Organism Klebsiella pneumoniae strain ENT1988

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag ICN80_RS24880 Protein ID WP_002882817.1
Coordinates 5170216..5170599 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag ICN80_RS24885 Protein ID WP_004150355.1
Coordinates 5170599..5170841 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ICN80_RS24865 5167582..5168484 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
ICN80_RS24870 5168481..5169116 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
ICN80_RS24875 5169113..5170042 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
ICN80_RS24880 5170216..5170599 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
ICN80_RS24885 5170599..5170841 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
ICN80_RS24890 5171046..5171963 + 918 WP_004213381.1 alpha/beta hydrolase -
ICN80_RS24895 5171978..5172919 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
ICN80_RS24900 5172964..5173401 - 438 WP_023302745.1 D-tyrosyl-tRNA(Tyr) deacylase -
ICN80_RS24905 5173398..5174258 - 861 WP_004146232.1 virulence factor BrkB family protein -
ICN80_RS24910 5174252..5174851 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T172467 WP_002882817.1 NZ_CP061350:c5170599-5170216 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T172467 NZ_CP079767:c2489640-2489242 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT172467 WP_004150355.1 NZ_CP061350:c5170841-5170599 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT172467 NZ_CP079767:c2489936-2489643 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References