Detailed information of TA system TA172412

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1835522..1836306
• Replicon: chromosome
• Accession: NZ_CP061339
• Organism: Escherichia coli strain M00057

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: IC755_RS08770
• Protein ID: WP_000613626.1
• Coordinates: 1835522..1836016 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: IC755_RS08775
• Protein ID: WP_001110447.1
• Coordinates: 1836013..1836306 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC755_RS08750	1830715..1831812	+	1098	WP_000589320.1	flagellar basal body P-ring protein FlgI	-
IC755_RS08755	1831812..1832753	+	942	WP_001309406.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
IC755_RS08760	1832819..1834462	+	1644	WP_000096524.1	flagellar hook-associated protein FlgK	-
IC755_RS08765	1834474..1835427	+	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
IC755_RS08770	1835522..1836016	-	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
IC755_RS08775	1836013..1836306	-	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
IC755_RS08780	1836439..1839624	-	3186	WP_000827427.1	ribonuclease E	-
IC755_RS08785	1840197..1841156	+	960	WP_000846342.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T172412 WP_000613626.1 NZ_CP061339:c1836016-1835522 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T172412 NZ_CP079752:c67391-67083 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGATCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTTTGACGGGATTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT172412 WP_001110447.1 NZ_CP061339:c1836306-1836013 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT172412 NZ_CP079752:c67630-67394 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6