Detailed information of TA system TA172404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 72555..73180
• Replicon: plasmid unnamed
• Accession: NZ_CP061338
• Organism: Escherichia coli strain M11957

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: IC756_RS23885
• Protein ID: WP_000911315.1
• Coordinates: 72555..72953 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: L4J1D2
• Locus tag: IC756_RS23890
• Protein ID: WP_000450532.1
• Coordinates: 72953..73180 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC756_RS23870	68809..69330	+	522	WP_000632670.1	conjugal transfer entry exclusion protein TraS	-
IC756_RS23875	69355..70086	+	732	WP_000850422.1	complement resistance protein TraT	-
IC756_RS23880	70339..72546	+	2208	WP_188926461.1	type IV conjugative transfer system coupling protein TraD	-
IC756_RS23885	72555..72953	-	399	WP_000911315.1	tRNA(fMet)-specific endonuclease VapC	Toxin
IC756_RS23890	72953..73180	-	228	WP_000450532.1	toxin-antitoxin system antitoxin VapB	Antitoxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / iroE	1..135788	135788

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14871.17 Da
• Isoelectric Point: 8.5264

>T172404 WP_000911315.1 NZ_CP061338:c72953-72555 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELARQARPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Nucleotide

• Length: 399 bp

>T172404 NZ_CP079748:1663033-1663314 [Acinetobacter johnsonii]
TTGATTAAGAGTTTTAAACATAAAGGTCTTCAGGCTTTTTTCCAAACAGGTACAACAGCAGGTATCCAAGCAACGCATTC
TTCTAAATTACGTTTGATTCTTGCGGCATTACATGCAGCGTCTACAGTGAATGATTTGAGGATACCACCCAATTGGCGCC
TACATAAGCTGAATGGGAATTTGCAAGATCAATGGTCACTGACCGTAAACGGTAATTGGCGGGTAATTTTTAAATTTGAA
GATGGCGATGTCTACATCGTGGATTATCTGGACTATCACTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8512.55 Da
• Isoelectric Point: 4.5358

>AT172404 WP_000450532.1 NZ_CP061338:c73180-72953 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Nucleotide

• Length: 228 bp

>AT172404 NZ_CP079748:1663333-1663617 [Acinetobacter johnsonii]
ATGAATATGATGTTTAATGCGCCTCATCCTGGCGAAATGTTGCGGGAATACATGGGTGAAACTCCTGTGACTGAGGCTGC
TCAAGCGCTAGGTGTTACTCGAGCAAATCTTTCTCGTATTTTGAATGGCCATACAGGTATTTCAGCAGATATGGCGATTC
GTTTAAGCGAGGCTTTAGAAACGTCACCAGAGTTTTGGTTGAATTTACAAATGCAGCATGATTTATGGAACGCAAGTCAG
CGTCAGCGACCAGTCATAAAGAAATTGGTTCAGGATTTTGCATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CHF5