Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 21551..22076 Replicon plasmid unnamed
Accession NZ_CP061338
Organism Escherichia coli strain M11957

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag IC756_RS23560 Protein ID WP_001159871.1
Coordinates 21771..22076 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag IC756_RS23555 Protein ID WP_000813639.1
Coordinates 21551..21769 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC756_RS23535 17652..17999 - 348 WP_000612591.1 IS66 family insertion sequence element accessory protein TnpB -
IC756_RS23540 17996..18376 - 381 WP_001171554.1 IS66 family insertion sequence hypothetical protein -
IC756_RS23545 18467..19882 - 1416 Protein_21 NTPase -
IC756_RS23550 19884..20972 - 1089 WP_000952217.1 transcriptional repressor PifC -
IC756_RS23555 21551..21769 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
IC756_RS23560 21771..22076 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
IC756_RS23565 22129..22861 + 733 Protein_25 phage integrase family protein -
IC756_RS23570 23648..24403 + 756 WP_000852143.1 replication initiation protein RepE -
IC756_RS23575 24991..26157 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / iroE 1..135788 135788


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T172403 WP_001159871.1 NZ_CP061338:21771-22076 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T172403 NZ_CP079747:c4071375-4071202 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT172403 WP_000813639.1 NZ_CP061338:21551-21769 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT172403 NZ_CP079747:c4071699-4071403 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References