Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3741793..3742447 Replicon chromosome
Accession NZ_CP061337
Organism Escherichia coli strain M11957

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag IC756_RS17880 Protein ID WP_000244777.1
Coordinates 3741793..3742200 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag IC756_RS17885 Protein ID WP_000354046.1
Coordinates 3742181..3742447 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IC756_RS17860 3737750..3739483 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
IC756_RS17865 3739489..3740199 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
IC756_RS17870 3740224..3741120 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
IC756_RS17875 3741232..3741753 + 522 WP_001055874.1 flavodoxin FldB -
IC756_RS17880 3741793..3742200 - 408 WP_000244777.1 toxin CptA Toxin
IC756_RS17885 3742181..3742447 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
IC756_RS17890 3742690..3743670 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
IC756_RS17895 3743866..3744525 - 660 WP_000250274.1 hemolysin III family protein -
IC756_RS17900 3744689..3745000 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
IC756_RS17905 3745045..3746478 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
IC756_RS17910 3746535..3747278 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T172398 WP_000244777.1 NZ_CP061337:c3742200-3741793 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172398 NZ_CP079747:3323070-3323288 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172398 WP_000354046.1 NZ_CP061337:c3742447-3742181 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172398 NZ_CP079747:3322670-3323044 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References