Detailed information of TA system TA172389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2234006..2235594
• Replicon: chromosome
• Accession: NZ_CP061337
• Organism: Escherichia coli strain M11957

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: U9YVT8
• Locus tag: IC756_RS10685
• Protein ID: WP_001125415.1
• Coordinates: 2234006..2235328 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: IC756_RS10690
• Protein ID: WP_001296726.1
• Coordinates: 2235328..2235594 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC756_RS10645	2229040..2229612	-	573	WP_000789913.1	NAD(P)H-dependent oxidoreductase	-
IC756_RS10650	2229763..2229954	-	192	WP_001469139.1	amidohydrolase family protein	-
IC756_RS10655	2229905..2230324	-	420	WP_001322416.1	amidohydrolase family protein	-
IC756_RS10660	2230284..2230439	-	156	WP_001322415.1	hypothetical protein	-
IC756_RS10665	2230542..2231489	-	948	WP_000854796.1	aldo/keto reductase	-
IC756_RS10670	2231563..2232252	-	690	WP_000801313.1	type 1 glutamine amidotransferase domain-containing protein	-
IC756_RS10675	2232261..2232569	-	309	WP_001221046.1	hypothetical protein	-
IC756_RS10680	2232709..2233608	+	900	WP_001057776.1	LysR family transcriptional regulator	-
IC756_RS10685	2234006..2235328	-	1323	WP_001125415.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
IC756_RS10690	2235328..2235594	-	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
IC756_RS10695	2235805..2237205	-	1401	WP_001376867.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49384.05 Da
• Isoelectric Point: 8.5830

>T172389 WP_001125415.1 NZ_CP061337:c2235328-2234006 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHRRLSREYGIK

Nucleotide

• Length: 1323 bp

>T172389 NZ_CP079747:671989-672303 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT172389 WP_001296726.1 NZ_CP061337:c2235594-2235328 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT172389 NZ_CP079747:672300-672716 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: U9YVT8