Detailed information of TA system TA172381

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1518901..1519606
• Replicon: chromosome
• Accession: NZ_CP061337
• Organism: Escherichia coli strain M11957

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: IC756_RS07110
• Protein ID: WP_000539521.1
• Coordinates: 1519220..1519606 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: IC756_RS07105
• Protein ID: WP_001280945.1
• Coordinates: 1518901..1519230 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IC756_RS07090	1514064..1514975	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
IC756_RS07095	1515153..1517495	+	2343	WP_001376787.1	EAL domain-containing protein	-
IC756_RS07100	1517503..1518831	+	1329	WP_000086879.1	GGDEF domain-containing protein	-
IC756_RS07105	1518901..1519230	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
IC756_RS07110	1519220..1519606	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IC756_RS07115	1519832..1521157	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
IC756_RS07120	1521370..1521753	+	384	WP_000497137.1	biofilm formation regulator BssR	-
IC756_RS07125	1521864..1522979	+	1116	WP_000555038.1	aldose sugar dehydrogenase YliI	-
IC756_RS07130	1522976..1523602	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T172381 WP_000539521.1 NZ_CP061337:c1519606-1519220 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T172381 NZ_CP079745:c618289-618035 [Comamonas aquatica]
ATGAGATTAATTTTTTCCGATGATGCATGGGAAGATTATCTGTTCTGGCAAAAACAAGACCGCCGCATGGTTGATCGAAT
TAACAAGCTAATTAAAGAAACCACCCGAGAGCCTTTCTCTGGAGTGGGCAAGCCAGAGCCATTAAAACATGCTTTGGCCG
GTTATTGGTCTCGTCGAATTACGGATGAGCACCGAATGGTTTACAAAGTGGCGGATGATGCCCTTTGGATCGTTCAACTC
AAGTACCACTACTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT172381 WP_001280945.1 NZ_CP061337:c1519230-1518901 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT172381 NZ_CP079745:c618537-618286 [Comamonas aquatica]
ATGGACGCTATCACTTACACACACGCCCGCGCCAACCTGGCTGGAACCATGGATCGTGTTTGCAACGATCATGAGCCGGT
GATCATCACACGTAACGGCGACCAATCGGTTGTGATTCTCTCGCTGGAAGATTTCCAGGCGCTGGAAGAAACTGCGTATC
TGCTGCGCAGCCCCGCCAACGCCAAGCGGCTTTTCAAGTCGATCGAGCAACTTGAAGCGGGCCGAGGACAGGCGCGGGAG
TTGGCCGAATGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6