Detailed information of TA system TA172374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 5185518..5186149
• Replicon: chromosome
• Accession: NZ_CP061335
• Organism: Pseudomonas asiatica strain C1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0C1KJT5
• Locus tag: ICJ54_RS23575
• Protein ID: WP_003258415.1
• Coordinates: 5185922..5186149 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ICJ54_RS23570
• Protein ID: WP_189121365.1
• Coordinates: 5185518..5185925 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICJ54_RS23550 (ICJ54_23550)	5182175..5183503	+	1329	WP_189121364.1	phosphatase PAP2/dual specificity phosphatase family protein	-
ICJ54_RS23555 (ICJ54_23555)	5183500..5183931	+	432	WP_015271816.1	hypothetical protein	-
ICJ54_RS23560 (ICJ54_23560)	5183942..5184565	+	624	WP_015271817.1	lysophospholipid acyltransferase family protein	-
ICJ54_RS23565 (ICJ54_23565)	5184567..5185499	+	933	WP_085720976.1	phosphatidate cytidylyltransferase	-
ICJ54_RS23570 (ICJ54_23570)	5185518..5185925	-	408	WP_189121365.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ICJ54_RS23575 (ICJ54_23575)	5185922..5186149	-	228	WP_003258415.1	type II toxin-antitoxin system HicA family toxin	Toxin
ICJ54_RS23580 (ICJ54_23580)	5186345..5186620	-	276	WP_015271819.1	metal/formaldehyde-sensitive transcriptional repressor	-
ICJ54_RS23585 (ICJ54_23585)	5186691..5187632	+	942	WP_189121366.1	CDF family Co(II)/Ni(II) efflux transporter DmeF	-
ICJ54_RS23590 (ICJ54_23590)	5187729..5188073	+	345	WP_003258412.1	cupin domain-containing protein	-
ICJ54_RS23595 (ICJ54_23595)	5188381..5188869	+	489	WP_023663075.1	type VI secretion system tube protein Hcp	-
ICJ54_RS23600 (ICJ54_23600)	5188880..5189857	+	978	WP_041505614.1	hypothetical protein	-
ICJ54_RS23605 (ICJ54_23605)	5189854..5190246	+	393	WP_143492656.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8270.38 Da
• Isoelectric Point: 10.9929

>T172374 WP_003258415.1 NZ_CP061335:c5186149-5185922 [Pseudomonas asiatica]
MRSREVIQLIEADGWYQVDVKGSHYQFRHPTKRGRVTVPHPKSELPKGTVRSILKQAGLIQANGTASTSINGGSQ

Nucleotide

• Length: 228 bp

>T172374 NZ_CP079736:c3995627-3995277 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14685.57 Da
• Isoelectric Point: 4.7475

>AT172374 WP_189121365.1 NZ_CP061335:c5185925-5185518 [Pseudomonas asiatica]
MKFPVVLHKDADSDYGVTVPDVPGCFSAGATVAKALENVQEALALHFEGLVADNEALPQAQEVDVHVANPDYEGGVWAVV
DFDVIPYLGKAVRFNATLPENLLQRIDEKVKRDHRYASRSGFLASAALRELALSH

Nucleotide

• Length: 408 bp

>AT172374 NZ_CP079736:c3995872-3995621 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A0C1KJT5