Detailed information of TA system TA172373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 3909949..3910832
• Replicon: chromosome
• Accession: NZ_CP061335
• Organism: Pseudomonas asiatica strain C1

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ICJ54_RS17825
• Protein ID: WP_013973162.1
• Coordinates: 3909949..3910335 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: F8G488
• Locus tag: ICJ54_RS17830
• Protein ID: WP_013973163.1
• Coordinates: 3910383..3910832 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICJ54_RS17805 (ICJ54_17805)	3905177..3906241	+	1065	WP_061304051.1	TerC family protein	-
ICJ54_RS17810 (ICJ54_17810)	3906319..3907548	-	1230	WP_013973159.1	acyl-CoA dehydrogenase	-
ICJ54_RS17815 (ICJ54_17815)	3907689..3908615	+	927	WP_013973160.1	LysR family transcriptional regulator	-
ICJ54_RS17820 (ICJ54_17820)	3908787..3909941	+	1155	WP_015270850.1	aminotransferase class V-fold PLP-dependent enzyme	-
ICJ54_RS17825 (ICJ54_17825)	3909949..3910335	-	387	WP_013973162.1	RES family NAD+ phosphorylase	Toxin
ICJ54_RS17830 (ICJ54_17830)	3910383..3910832	-	450	WP_013973163.1	DUF2384 domain-containing protein	Antitoxin
ICJ54_RS17835 (ICJ54_17835)	3910931..3911584	-	654	WP_054572086.1	oxygen-insensitive NAD(P)H-dependent nitroreductase NfsB	-
ICJ54_RS17840 (ICJ54_17840)	3911689..3912612	+	924	WP_015270853.1	LysR family transcriptional regulator	-
ICJ54_RS17845 (ICJ54_17845)	3912660..3913490	+	831	WP_015270854.1	AraC family transcriptional regulator	-
ICJ54_RS17850 (ICJ54_17850)	3913541..3914125	+	585	WP_189121233.1	LysE family translocator	-
ICJ54_RS17855 (ICJ54_17855)	3914265..3915464	-	1200	WP_061304062.1	sugar transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-120)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (33-91)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14226.21 Da
• Isoelectric Point: 5.0980

>T172373 WP_013973162.1 NZ_CP061335:c3910335-3909949 [Pseudomonas asiatica]
MRVSGRWHQAGRPVVYAATSPAGAMLEVLVHLEIDPEDFPTTMRLLRIELPDTVSQAQLPALQPGWSSQTELTRGLGNRF
LDECSALLLPVPSAIMPSTTNYLFNPRHPQANSARLEVEDFTPDSRLF

Nucleotide

• Length: 387 bp

>T172373 NZ_CP079736:3908820-3909077 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 17004.59 Da
• Isoelectric Point: 6.3405

>AT172373 WP_013973163.1 NZ_CP061335:c3910832-3910383 [Pseudomonas asiatica]
MLAEVLRDNGYHEYRARLHALLEIPELASDFEIHTRITHGFAATWLVKLTELGVFTPVERDQIIPLRTLKTRIERDQPLT
VEESDRLFRSAHITAMAEAVFGEAGKAKRWLSKPKERFSGLTPMQMLTTQQGTTQVEEMLLQIAEGFGL

Nucleotide

• Length: 450 bp

>AT172373 NZ_CP079736:3909089-3909634 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Structures

Antitoxin

• AlphaFold DB: F8G488