Detailed information of TA system TA172372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 2602376..2602892
• Replicon: chromosome
• Accession: NZ_CP061335
• Organism: Pseudomonas asiatica strain C1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F8G1E7
• Locus tag: ICJ54_RS11915
• Protein ID: WP_003259987.1
• Coordinates: 2602611..2602892 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F8G1E6
• Locus tag: ICJ54_RS11910
• Protein ID: WP_013972043.1
• Coordinates: 2602376..2602621 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICJ54_RS11885 (ICJ54_11885)	2597788..2598267	-	480	WP_085721567.1	transposase	-
ICJ54_RS11890 (ICJ54_11890)	2598524..2599066	-	543	WP_015269935.1	WYL domain-containing protein	-
ICJ54_RS11900 (ICJ54_11900)	2599802..2600995	+	1194	WP_025338595.1	MFS transporter	-
ICJ54_RS11905 (ICJ54_11905)	2601026..2602132	+	1107	WP_189121758.1	alkene reductase	-
ICJ54_RS11910 (ICJ54_11910)	2602376..2602621	+	246	WP_013972043.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ICJ54_RS11915 (ICJ54_11915)	2602611..2602892	+	282	WP_003259987.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICJ54_RS11920 (ICJ54_11920)	2602932..2603327	-	396	WP_023662326.1	hypothetical protein	-
ICJ54_RS11925 (ICJ54_11925)	2603416..2604324	-	909	WP_013972045.1	LysR family transcriptional regulator	-
ICJ54_RS11930 (ICJ54_11930)	2604675..2605919	+	1245	WP_263407088.1	MFS transporter	-
ICJ54_RS11935 (ICJ54_11935)	2605950..2607191	+	1242	WP_189121759.1	Zn-dependent hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (10-76)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10909.78 Da
• Isoelectric Point: 10.7594

>T172372 WP_003259987.1 NZ_CP061335:2602611-2602892 [Pseudomonas asiatica]
MTYKLEFLPSALKEWGKLGHTVREQIKKKLAERLQSPKVQADALRDLPNHYKIKLKASGYRLVYRVEDERIVVVVVSVGK
RERSEVYRSAQKR

Nucleotide

• Length: 282 bp

>T172372 NZ_CP079736:3634893-3635171 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8779.21 Da
• Isoelectric Point: 4.3611

>AT172372 WP_013972043.1 NZ_CP061335:2602376-2602621 [Pseudomonas asiatica]
MQNIFADVAVSVSELKKNPSAVMTGAAGMPVAVLNHNRVMGYMVPAELYEAMMERLEDLELSELAKSRLGEKGVPVSLDD
L

Nucleotide

• Length: 246 bp

>AT172372 NZ_CP079736:3634630-3634890 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Structures

Toxin

• AlphaFold DB: F8G1E7

Antitoxin

• AlphaFold DB: F8G1E6