Detailed information of TA system TA172371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1391012..1391598
• Replicon: chromosome
• Accession: NZ_CP061335
• Organism: Pseudomonas asiatica strain C1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: ICJ54_RS06340
• Protein ID: WP_080604915.1
• Coordinates: 1391012..1391305 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A2N5BNF2
• Locus tag: ICJ54_RS06345
• Protein ID: WP_061303299.1
• Coordinates: 1391302..1391598 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICJ54_RS06315 (ICJ54_06315)	1386545..1387399	+	855	WP_085720784.1	putative selenate ABC transporter substrate-binding protein	-
ICJ54_RS06320 (ICJ54_06320)	1387420..1388214	+	795	WP_061303320.1	phosphonate ABC transporter ATP-binding protein	-
ICJ54_RS06325 (ICJ54_06325)	1388208..1389035	+	828	WP_085720783.1	ABC transporter permease	-
ICJ54_RS06330 (ICJ54_06330)	1389032..1389799	+	768	WP_015269003.1	phosphonate ABC transporter, permease protein PhnE	-
ICJ54_RS06335 (ICJ54_06335)	1389905..1390791	-	887	Protein_1237	effector protein	-
ICJ54_RS06340 (ICJ54_06340)	1391012..1391305	+	294	WP_080604915.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICJ54_RS06345 (ICJ54_06345)	1391302..1391598	+	297	WP_061303299.1	putative addiction module antidote protein	Antitoxin
ICJ54_RS06350 (ICJ54_06350)	1391752..1392654	-	903	WP_015269007.1	transporter substrate-binding domain-containing protein	-
ICJ54_RS06355 (ICJ54_06355)	1392682..1393953	-	1272	WP_015269008.1	D-amino acid dehydrogenase	-
ICJ54_RS06360 (ICJ54_06360)	1394061..1394966	+	906	WP_015269009.1	LysR substrate-binding domain-containing protein	-
ICJ54_RS06370 (ICJ54_06370)	1395208..1396257	+	1050	WP_013971008.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-86)

Antitoxin

• Uncharacterized protein HI1420 (4-92)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10793.69 Da
• Isoelectric Point: 10.5815

>T172371 WP_080604915.1 NZ_CP061335:1391012-1391305 [Pseudomonas asiatica]
MYLVEQTESFAVWLSSLRDLRAQLAIGRRLERAAMGNLGDVKWLGGGIGELRIDVAAGYRVYFAQKGQRLVLLLVGGDKS
TQATDILKARKLLKEMK

Nucleotide

• Length: 294 bp

>T172371 NZ_CP079736:c3628831-3628433 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10658.38 Da
• Isoelectric Point: 6.4669

>AT172371 WP_061303299.1 NZ_CP061335:1391302-1391598 [Pseudomonas asiatica]
MSEKLIPFDMAALLSSDEAITEYLSQVLADGDTEEIIRALGHIARARGMTRIATESGLGRESLYKALSPGAKPRFDTVLK
VIRALGVDLLVQPHVVPR

Nucleotide

• Length: 297 bp

>AT172371 NZ_CP079736:c3629127-3628834 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2N5BNF2