Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4349887..4350384 Replicon chromosome
Accession NZ_CP061329
Organism Escherichia coli strain EC93

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H8529_RS20880 Protein ID WP_001173343.1
Coordinates 4349887..4350060 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H8529_RS20885 Protein ID WP_000398619.1
Coordinates 4350088..4350384 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8529_RS20870 4346557..4348014 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
H8529_RS20875 4348251..4349768 + 1518 WP_001295074.1 lysine--tRNA ligase -
H8529_RS20880 4349887..4350060 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H8529_RS20885 4350088..4350384 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H8529_RS20890 4350611..4350883 - 273 WP_000405647.1 N-acetyltransferase -
H8529_RS20895 4350895..4351125 - 231 WP_000371700.1 (4Fe-4S)-binding protein -
H8529_RS20900 4351306..4352937 + 1632 WP_001216460.1 sensor histidine kinase -
H8529_RS20905 4352934..4353653 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T172368 WP_001173343.1 NZ_CP061329:c4350060-4349887 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T172368 NZ_CP079736:2656998-2657105 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT172368 WP_000398619.1 NZ_CP061329:c4350384-4350088 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT172368 NZ_CP079736:c2656951-2656885 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References