Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1300528..1301153 Replicon chromosome
Accession NZ_CP061329
Organism Escherichia coli strain EC93

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag H8529_RS06260 Protein ID WP_000911329.1
Coordinates 1300755..1301153 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag H8529_RS06255 Protein ID WP_000450524.1
Coordinates 1300528..1300755 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8529_RS06230 1296330..1296800 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
H8529_RS06235 1296800..1297372 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
H8529_RS06240 1297518..1298396 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
H8529_RS06245 1298413..1299447 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
H8529_RS06250 1299660..1300373 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
H8529_RS06255 1300528..1300755 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
H8529_RS06260 1300755..1301153 + 399 WP_000911329.1 tRNA(fMet)-specific endonuclease VapC Toxin
H8529_RS06265 1301300..1302163 + 864 WP_001267498.1 neutral zinc metallopeptidase -
H8529_RS06270 1302178..1304193 + 2016 WP_188214209.1 tRNA cytosine(34) acetyltransferase TmcA -
H8529_RS06275 1304267..1304965 + 699 WP_000679823.1 esterase -
H8529_RS06280 1305075..1305275 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T172354 WP_000911329.1 NZ_CP061329:1300755-1301153 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T172354 NZ_CP079736:653232-653546 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT172354 WP_000450524.1 NZ_CP061329:1300528-1300755 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT172354 NZ_CP079736:653543-653959 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References