Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 871360..872014 Replicon chromosome
Accession NZ_CP061329
Organism Escherichia coli strain EC93

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H8529_RS04220 Protein ID WP_000244772.1
Coordinates 871607..872014 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H8529_RS04215 Protein ID WP_000354046.1
Coordinates 871360..871626 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8529_RS04190 866648..867391 + 744 WP_000951964.1 SDR family oxidoreductase -
H8529_RS04195 867448..868881 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H8529_RS04200 868926..869237 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
H8529_RS04205 869401..870060 + 660 WP_000250274.1 hemolysin III family protein -
H8529_RS04210 870137..871117 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H8529_RS04215 871360..871626 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H8529_RS04220 871607..872014 + 408 WP_000244772.1 protein YgfX Toxin
H8529_RS04225 872054..872575 - 522 WP_001055874.1 flavodoxin FldB -
H8529_RS04230 872687..873583 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H8529_RS04235 873608..874318 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H8529_RS04240 874324..876057 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T172352 WP_000244772.1 NZ_CP061329:871607-872014 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T172352 NZ_CP079736:184820-184927 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT172352 WP_000354046.1 NZ_CP061329:871360-871626 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT172352 NZ_CP079736:c184772-184706 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References