Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 279885..280516 Replicon chromosome
Accession NZ_CP061329
Organism Escherichia coli strain EC93

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag H8529_RS01240 Protein ID WP_001259388.1
Coordinates 279885..280160 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H8529_RS01245 Protein ID WP_000593555.1
Coordinates 280157..280516 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H8529_RS01225 274889..275956 + 1068 WP_000361467.1 HlyD family secretion protein -
H8529_RS01230 275953..278688 + 2736 WP_000149124.1 ribosome-associated ATPase/putative transporter RbbA -
H8529_RS01235 278688..279812 + 1125 WP_001216257.1 ABC transporter permease -
H8529_RS01240 279885..280160 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
H8529_RS01245 280157..280516 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H8529_RS01250 280636..281037 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H8529_RS01255 281043..281849 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H8529_RS01260 281846..282610 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H8529_RS01265 282610..283443 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
H8529_RS01270 283440..284384 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 272901..281037 8136


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T172350 WP_001259388.1 NZ_CP061329:279885-280160 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T172350 NZ_CP079735:c3994534-3994184 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT172350 WP_000593555.1 NZ_CP061329:280157-280516 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT172350 NZ_CP079735:c3994779-3994528 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References