Detailed information of TA system TA172348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1526319..1526943
• Replicon: chromosome
• Accession: NZ_CP061319
• Organism: Polynucleobacter sp. AM-7D1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GQ359_RS08060
• Protein ID: WP_215386531.1
• Coordinates: 1526539..1526943 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GQ359_RS08055
• Protein ID: WP_215386529.1
• Coordinates: 1526319..1526549 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ359_RS08030 (GQ359_08035)	1521676..1522137	-	462	WP_215386521.1	DUF6306 domain-containing protein	-
GQ359_RS08035 (GQ359_08040)	1522363..1523055	-	693	WP_215386523.1	DUF2461 family protein	-
GQ359_RS08040 (GQ359_08045)	1523292..1524002	-	711	WP_215386525.1	esterase	-
GQ359_RS08045 (GQ359_08050)	1523995..1525650	-	1656	WP_215386527.1	AAA family ATPase	-
GQ359_RS08055 (GQ359_08060)	1526319..1526549	+	231	WP_215386529.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GQ359_RS08060 (GQ359_08065)	1526539..1526943	+	405	WP_215386531.1	PIN domain-containing protein	Toxin
GQ359_RS08065 (GQ359_08070)	1526972..1529515	-	2544	WP_215386533.1	RNA polymerase sigma factor RpoD	-
GQ359_RS08070 (GQ359_08075)	1529739..1531682	-	1944	WP_215387941.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-122)

Antitoxin

• SpoVT-AbrB domain (5-39)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15592.80 Da
• Isoelectric Point: 6.7183

>T172348 WP_215386531.1 NZ_CP061319:1526539-1526943 [Polynucleobacter sp. AM-7D1]
MNAKKIFFDTNTLLYLLSSDNKKADWVSKNLQQHNVISVQVLNEFTSASLRKIKISNTELDEFLDLFTSTFQVRSLDMDT
FETGLMVSRRYGYQHYDSMIIAAALQAGCERLYSEDMQHRQVIDKRLQIVNPFV

Nucleotide

• Length: 405 bp

>T172348 NZ_CP079735:3633804-3634082 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 9006.41 Da
• Isoelectric Point: 10.3404

>AT172348 WP_215386529.1 NZ_CP061319:1526319-1526549 [Polynucleobacter sp. AM-7D1]
MIVSKWGNSLAIRLPSQVVDYLKLKEGDDIEVHVTDKKHFQIKKKPSLQERIEGLRKFRGRMPADFHFDRSELNER

Nucleotide

• Length: 231 bp

>AT172348 NZ_CP079735:3633541-3633801 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA