Detailed information of TA system TA172346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 554216..554805
• Replicon: chromosome
• Accession: NZ_CP061319
• Organism: Polynucleobacter sp. AM-7D1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GQ359_RS02910
• Protein ID: WP_215387428.1
• Coordinates: 554216..554599 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GQ359_RS02915
• Protein ID: WP_215387429.1
• Coordinates: 554596..554805 (-)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQ359_RS02895 (GQ359_02895)	549778..550896	+	1119	WP_215387425.1	alanine dehydrogenase	-
GQ359_RS02900 (GQ359_02900)	550969..551544	+	576	WP_215387426.1	TMEM165/GDT1 family protein	-
GQ359_RS02905 (GQ359_02905)	551579..554188	+	2610	WP_215387427.1	aminopeptidase N	-
GQ359_RS02910 (GQ359_02910)	554216..554599	-	384	WP_215387428.1	VapC toxin family PIN domain ribonuclease	Toxin
GQ359_RS02915 (GQ359_02915)	554596..554805	-	210	WP_215387429.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GQ359_RS02920 (GQ359_02920)	555025..556011	+	987	WP_215387871.1	class 1 fructose-bisphosphatase	-
GQ359_RS02925 (GQ359_02925)	556110..558680	-	2571	WP_215387430.1	cyanophycin synthetase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14064.30 Da
• Isoelectric Point: 5.5857

>T172346 WP_215387428.1 NZ_CP061319:c554599-554216 [Polynucleobacter sp. AM-7D1]
MTMVLADTSVWISHFRKSNPSLQSLLVNDQILCHPLVLLELSCGSPPAPKLTTLSQLKSLRQATIATTEETIDFIEKNKF
YDLGCGAVDISLLASTLLSKNALLWTFDKNLEALATQLDVAFKENFH

Nucleotide

• Length: 384 bp

>T172346 NZ_CP079735:3617150-3617491 [Escherichia coli]
ATGAAAACTTTACCTGCAATAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 7817.06 Da
• Isoelectric Point: 10.2690

>AT172346 WP_215387429.1 NZ_CP061319:c554805-554596 [Polynucleobacter sp. AM-7D1]
MRTTVTIDDALYQRGLELADPEMDKADLFREAMNVFVRVQIGKRLAALGGKAPEMKNIPRRKPKDSQQS

Nucleotide

• Length: 210 bp

>AT172346 NZ_CP079735:3616812-3617129 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCTCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA