Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 124589..125127 Replicon chromosome
Accession NZ_CP061316
Organism Polynucleobacter sp. AP-Jannik-300A-C4

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag FD975_RS00730 Protein ID WP_215302398.1
Coordinates 124589..124915 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag FD975_RS00735 Protein ID WP_215302399.1
Coordinates 124912..125127 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FD975_RS00705 (FD975_00705) 120614..120922 - 309 WP_215302393.1 Grx4 family monothiol glutaredoxin -
FD975_RS00710 (FD975_00710) 121027..121875 - 849 WP_215302394.1 peptide chain release factor N(5)-glutamine methyltransferase -
FD975_RS00715 (FD975_00715) 121886..122965 - 1080 WP_215302395.1 peptide chain release factor 1 -
FD975_RS00720 (FD975_00720) 122966..124282 - 1317 WP_215302396.1 glutamyl-tRNA reductase -
FD975_RS00725 (FD975_00725) 124284..124592 + 309 WP_215302397.1 hypothetical protein -
FD975_RS00730 (FD975_00730) 124589..124915 - 327 WP_215302398.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
FD975_RS00735 (FD975_00735) 124912..125127 - 216 WP_215302399.1 antitoxin MazE family protein Antitoxin
FD975_RS00740 (FD975_00740) 125196..125966 - 771 WP_215302400.1 uracil-DNA glycosylase -
FD975_RS00745 (FD975_00745) 125966..127750 - 1785 WP_215302401.1 PDZ domain-containing protein -
FD975_RS00750 (FD975_00750) 127769..128488 - 720 WP_251371205.1 DsbC family protein -
FD975_RS00755 (FD975_00755) 128481..129755 - 1275 WP_215302402.1 FAD-dependent monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(8-69)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11785.77 Da        Isoelectric Point: 7.0124

>T172338 WP_215302398.1 NZ_CP061316:c124915-124589 [Polynucleobacter sp. AP-Jannik-300A-C4]
MKRGNVVTVAMQGDFGKPRPALVLQSDVFSDIHATVTVALISSEIVQAPIFRLDIEPNETNGLSRPSQVQIDKIMSIRSE
KIGSVIGELNDVMMVRVNRALALWLGLA

Download         Length: 327 bp

>T172338 NZ_CP079735:c1803001-1802627 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 8075.15 Da        Isoelectric Point: 5.7784

>AT172338 WP_215302399.1 NZ_CP061316:c125127-124912 [Polynucleobacter sp. AP-Jannik-300A-C4]
MSSNISLRVQKHRLALRDAGLRPIQIWVPNTQAPGFFEECRRQSALVARSDRADKELMSFLDDALADFDEA

Download         Length: 216 bp

>AT172338 NZ_CP079735:c1803458-1803090 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References