Detailed information of TA system TA172334

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 275646..276245
• Replicon: chromosome
• Accession: NZ_CP061315
• Organism: Polynucleobacter sp. AP-Kolm-20A-A1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: C2745_RS01485
• Protein ID: WP_215384584.1
• Coordinates: 275646..275942 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C2745_RS01490
• Protein ID: WP_215384585.1
• Coordinates: 275943..276245 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2745_RS01460 (C2745_01460)	271817..272641	-	825	WP_215384579.1	energy transducer TonB	-
C2745_RS01465 (C2745_01465)	272668..273087	-	420	WP_215384580.1	protein TolR	-
C2745_RS01470 (C2745_01470)	273099..273755	-	657	WP_215384581.1	protein TolQ	-
C2745_RS01475 (C2745_01475)	273832..275076	+	1245	WP_215384582.1	serine hydroxymethyltransferase	-
C2745_RS01480 (C2745_01480)	275102..275548	+	447	WP_215384583.1	transcriptional regulator NrdR	-
C2745_RS01485 (C2745_01485)	275646..275942	+	297	WP_215384584.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2745_RS01490 (C2745_01490)	275943..276245	+	303	WP_215384585.1	putative addiction module antidote protein	Antitoxin
C2745_RS01495 (C2745_01495)	276252..277217	-	966	WP_215384586.1	GDP-mannose 4,6-dehydratase	-
C2745_RS01500 (C2745_01500)	277228..277893	-	666	WP_215384587.1	adenylate kinase	-
C2745_RS01505 (C2745_01505)	278007..278771	-	765	WP_215384588.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
C2745_RS01510 (C2745_01510)	278783..278962	-	180	WP_215384589.1	Trm112 family protein	-
C2745_RS01515 (C2745_01515)	279049..280146	-	1098	WP_215384590.1	tetraacyldisaccharide 4'-kinase	-
C2745_RS01520 (C2745_01520)	280158..280625	-	468	WP_215384591.1	biopolymer transporter ExbD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-93)

Antitoxin

• Uncharacterized protein HI1420 (13-98)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11206.08 Da
• Isoelectric Point: 9.9398

>T172334 WP_215384584.1 NZ_CP061315:275646-275942 [Polynucleobacter sp. AP-Kolm-20A-A1]
MYEIKKTDEFENWFSGIRDPLTRGRLLARLRKASLGSLGDIASVGDGVWEMREHFGAGYRMYYVSYRRTIILMLGGGCKS
TQIADIKKVKKLLLTLED

Nucleotide

• Length: 297 bp

>T172334 NZ_CP079735:c1118880-1118551 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11013.95 Da
• Isoelectric Point: 10.3962

>AT172334 WP_215384585.1 NZ_CP061315:275943-276245 [Polynucleobacter sp. AP-Kolm-20A-A1]
MKSVKKTKVKDSKKFDVVDYLKTEKDIAAYLSAVLEDGDPALFVAAIGDIARAKGMTEIAKKSGVTRESLYRALKIEARP
RFETVTRVVQALGMKLSVHA

Nucleotide

• Length: 303 bp

>AT172334 NZ_CP079735:c1119218-1118901 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA