Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-MazE
Location 125655..126193 Replicon chromosome
Accession NZ_CP061314
Organism Polynucleobacter corsicus strain AP-Melu-1000-A1

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag C2747_RS00740 Protein ID WP_215331829.1
Coordinates 125655..125981 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag C2747_RS00745 Protein ID WP_215331830.1
Coordinates 125978..126193 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C2747_RS00715 (C2747_00715) 121682..121990 - 309 WP_046329433.1 Grx4 family monothiol glutaredoxin -
C2747_RS00720 (C2747_00720) 122093..122941 - 849 WP_215331825.1 peptide chain release factor N(5)-glutamine methyltransferase -
C2747_RS00725 (C2747_00725) 122953..124032 - 1080 WP_215331826.1 peptide chain release factor 1 -
C2747_RS00730 (C2747_00730) 124033..125349 - 1317 WP_215331827.1 glutamyl-tRNA reductase -
C2747_RS00735 (C2747_00735) 125392..125649 + 258 WP_215331828.1 hypothetical protein -
C2747_RS00740 (C2747_00740) 125655..125981 - 327 WP_215331829.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
C2747_RS00745 (C2747_00745) 125978..126193 - 216 WP_215331830.1 antitoxin MazE family protein Antitoxin
C2747_RS00750 (C2747_00750) 126264..127034 - 771 WP_215331831.1 uracil-DNA glycosylase -
C2747_RS00755 (C2747_00755) 127034..128818 - 1785 WP_215331832.1 M61 family peptidase -
C2747_RS00760 (C2747_00760) 128831..129547 - 717 WP_251374772.1 DsbC family protein -
C2747_RS00765 (C2747_00765) 129540..130814 - 1275 WP_215331834.1 FAD-dependent monooxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-104)

Antitoxin

Antitoxin MazE-like

(8-69)


Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11711.77 Da        Isoelectric Point: 8.8093

>T172330 WP_215331829.1 NZ_CP061314:c125981-125655 [Polynucleobacter corsicus]
MKRGNVVTVAMQGDFGKPRPALVLQSDVFSDIHATVTIALISSNLMQAPIFRLDIEPDGSNGLTKPSQVQIDKIQSIRIE
KIGAIIGELSDVMMVRVNRALALWLGLA

Download         Length: 327 bp

>T172330 NZ_CP079735:653230-653544 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7878.90 Da        Isoelectric Point: 4.4702

>AT172330 WP_215331830.1 NZ_CP061314:c126193-125978 [Polynucleobacter corsicus]
MPSNAALRVQKHRLALRDAGLRPIQIWVPDTQAPGFFEECRRQSALVAAADRADNELMAFLDDSLADLDEA

Download         Length: 216 bp

>AT172330 NZ_CP079735:653541-653957 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References