Detailed information of TA system TA172327

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1524611..1525235
• Replicon: chromosome
• Accession: NZ_CP061310
• Organism: Polynucleobacter sp. es-EL-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: FD974_RS07865
• Protein ID: WP_215364098.1
• Coordinates: 1524831..1525235 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FD974_RS07860
• Protein ID: WP_215364097.1
• Coordinates: 1524611..1524841 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FD974_RS07850 (FD974_07845)	1521023..1522234	-	1212	WP_215364094.1	restriction endonuclease subunit S	-
FD974_RS07855 (FD974_07850)	1522234..1523859	-	1626	WP_215364096.1	class I SAM-dependent DNA methyltransferase	-
FD974_RS07860 (FD974_07855)	1524611..1524841	+	231	WP_215364097.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FD974_RS07865 (FD974_07860)	1524831..1525235	+	405	WP_215364098.1	PIN domain-containing protein	Toxin
FD974_RS07870 (FD974_07865)	1525265..1527856	-	2592	WP_251374572.1	RNA polymerase sigma factor RpoD	-
FD974_RS07875 (FD974_07870)	1528081..1530042	-	1962	WP_215364099.1	DNA primase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1511791..1525235	13444

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-122)

Antitoxin

• SpoVT-AbrB domain (5-49)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 15474.65 Da
• Isoelectric Point: 6.7183

>T172327 WP_215364098.1 NZ_CP061310:1524831-1525235 [Polynucleobacter sp. es-EL-1]
MNAKKIFFDTNTLLYLLSSDNKKADWVSKNLQQHNVISVQVLNEFTSASLRKIKISNAELDEFLDLFTSTFNVRSLDIDT
FETGLMVSRRYGYQHYDSMIIAAALQAGCERLYSEDMQHRQVIDKKLQIVNPFA

Nucleotide

• Length: 405 bp

>T172327 NZ_CP079727:58939-59244 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8986.38 Da
• Isoelectric Point: 10.5573

>AT172327 WP_215364097.1 NZ_CP061310:1524611-1524841 [Polynucleobacter sp. es-EL-1]
MIVSKWGNSLAIRLPSQVVEFLKLKEGDDIEVQVADKKHFHIRKKPSPQERIEGLRKFRGRMPADFHFDRSELNER

Nucleotide

• Length: 231 bp

>AT172327 NZ_CP079727:58719-58937 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA