Detailed information of TA system TA172325

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-PumB/upstrm_HI1419-dnstrm_HI1420
• Location: 1165949..1166556
• Replicon: chromosome
• Accession: NZ_CP061306
• Organism: Polynucleobacter sp. JS-JIR-II-b4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: ICV90_RS05900
• Protein ID: WP_215356993.1
• Coordinates: 1165949..1166239 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: ICV90_RS05905
• Protein ID: WP_215356995.1
• Coordinates: 1166248..1166556 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ICV90_RS05885 (ICV90_05890)	1162058..1163199	+	1142	WP_215356987.1	IS3 family transposase	-
ICV90_RS05890 (ICV90_05895)	1163857..1164984	+	1128	WP_215356989.1	quinolinate synthase NadA	-
ICV90_RS05895 (ICV90_05900)	1165020..1165874	+	855	WP_215356991.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ICV90_RS05900 (ICV90_05905)	1165949..1166239	+	291	WP_215356993.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ICV90_RS05905 (ICV90_05910)	1166248..1166556	+	309	WP_215356995.1	putative addiction module antidote protein	Antitoxin
ICV90_RS05910 (ICV90_05915)	1166563..1167942	-	1380	WP_215356997.1	TAXI family TRAP transporter solute-binding subunit	-
ICV90_RS05915 (ICV90_05920)	1168010..1169668	-	1659	WP_215356999.1	L-aspartate oxidase	-
ICV90_RS05920 (ICV90_05925)	1169709..1169987	-	279	WP_215357009.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ICV90_RS05925 (ICV90_05930)	1169987..1170214	-	228	WP_215357011.1	ParD-like family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-95)

Antitoxin

• Uncharacterized protein HI1420 (7-94)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11046.82 Da
• Isoelectric Point: 10.4247

>T172325 WP_215356993.1 NZ_CP061306:1165949-1166239 [Polynucleobacter sp. JS-JIR-II-b4]
MHEIRKTEEFKSWLDSLNDLMGRAKIQARIKRLAEGNSGNTKSVGSKVFEMKIDFGPGYRIYYTKHQSAIYLLLIGGDKR
TQSKDIQIAKYLAKHI

Nucleotide

• Length: 291 bp

>T172325 NZ_CP079727:42826-43149 [Klebsiella pneumoniae]
ATGAACTATCTGGAGTTTGTTGAAACCAGCGCTTTCAGTGGACTGCGTAAGGAACTCATGGATGATGATGAATTCCGCGA
ACTGCAGACCTATTTACTGGAAGCCCATGATCGGGGTGACACCATCAGTCATACCGGTGGATGCAGGAAGATTCGCTGGG
GTCGCCCGGGGATGGGTAAGCGAGGTGGCGTCAGGGTGATTTACTACGTTCGTCTTGCCAGCGGCAGACTGTATCTGCTG
CTGATATACCCGAAGAATGCGAAAGATGAGCTCAGCGAGAAAGAAAAAGCCGTTATGAAGGCATTAACACAGCAGCTGAA
GTAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 10990.80 Da
• Isoelectric Point: 9.8362

>AT172325 WP_215356995.1 NZ_CP061306:1166248-1166556 [Polynucleobacter sp. JS-JIR-II-b4]
MKKTVTSPYDVAEHLRTPKEMSLYLQACIEESNGDAAFIAKALGDIARAKGMAQVAKESGLSRESLYKALSGDRIPGFDT
VLKVLTALGLSLHTKPISKIRA

Nucleotide

• Length: 309 bp

>AT172325 NZ_CP079727:43198-43500 [Klebsiella pneumoniae]
ATGGAAAAGAAACTTTTTGATGCCCTGATCAGCAGCATGGAGGAAATGGTGGCCATTGAAAATGGCCAGATCCAGCCCGC
TGCAGAACAGGTTCACCGCCATACACTGCCGGATGTGAAAGCCATTCGTAAACATGCCGGCATGAAACAGGCTGAATTTG
CAGAAGTGGTGGGGGCCAGCGTTGACCTTGTTCGCAGCTGGGAACAGCAACGGCGTGTTCCGTCGGGTATTGCGCTGAAA
ATGCTCTGCCTCATCGAGCGTGAGCCTGCACTGATTAATACGTTGCGTGGTATTCACGTCTGA