Detailed information of TA system TA172322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1139821..1140431
• Replicon: chromosome
• Accession: NZ_CP061302
• Organism: Polynucleobacter sp. MG-Unter2-18

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: C2759_RS05985
• Protein ID: WP_215353852.1
• Coordinates: 1139821..1140120 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: C2759_RS05990
• Protein ID: WP_251366917.1
• Coordinates: 1140117..1140431 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C2759_RS05965 (C2759_05960)	1135593..1136861	-	1269	WP_215353849.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
C2759_RS05970 (C2759_05965)	1136880..1138124	-	1245	WP_256441195.1	23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN	-
C2759_RS05975 (C2759_05970)	1138171..1138596	-	426	WP_046330292.1	nucleoside-diphosphate kinase	-
C2759_RS05980 (C2759_05975)	1138891..1139592	+	702	WP_215353850.1	Bax inhibitor-1 family protein	-
C2759_RS05985 (C2759_05980)	1139821..1140120	+	300	WP_215353852.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C2759_RS05990 (C2759_05985)	1140117..1140431	+	315	WP_251366917.1	putative addiction module antidote protein	Antitoxin
C2759_RS05995 (C2759_05990)	1140499..1141932	-	1434	WP_215353853.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
C2759_RS06000 (C2759_05995)	1141925..1142746	-	822	WP_215353854.1	3'-5' exonuclease	-
C2759_RS06005 (C2759_06000)	1142778..1143548	-	771	WP_251366918.1	peptidoglycan DD-metalloendopeptidase family protein	-
C2759_RS06010 (C2759_06005)	1143557..1144180	-	624	Protein_1168	protein-L-isoaspartate(D-aspartate) O-methyltransferase	-
C2759_RS06015 (C2759_06010)	1144210..1145004	-	795	WP_215353857.1	5'/3'-nucleotidase SurE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-95)

Antitoxin

• Uncharacterized protein HI1420 (18-102)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11387.28 Da
• Isoelectric Point: 10.0607

>T172322 WP_215353852.1 NZ_CP061302:1139821-1140120 [Polynucleobacter sp. MG-Unter2-18]
MYTVKETPEFQDWLDNIRDLKTRIRLAKRLVKVRNGTMGDIEPVGDGISEMREHFGPGWRMYYIKQGKVIIVMLGGGDKS
TQTADIKAVKRMLKTLEES

Nucleotide

• Length: 300 bp

>T172322 NZ_CP079725:c5251458-5251075 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11416.35 Da
• Isoelectric Point: 10.0312

>AT172322 WP_251366917.1 NZ_CP061302:1140117-1140431 [Polynucleobacter sp. MG-Unter2-18]
MKAVKEIKKTKIDALPNFDMARYLNSDKAITEYLNQVLEEGDDALLAEALGDIARAKGMAQIAQTTGITREALYKALRPN
AKPRFDTISKVIGALGIKLVAQRI

Nucleotide

• Length: 315 bp

>AT172322 NZ_CP079725:c5251700-5251458 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA